Hui Peng1, Lennart Salmén2, Jasna S Stevanic3, Jianxiong Lu4. 1. Research Institute of Wood Industry of Chinese Academy of Forestry, Hunan Collaborative Innovation Center for Effective Utilizing of Wood and Bamboo Resources, Beijing, 100091, People's Republic of China. 2. RISE Bioeconomy, Box 5604, 11486, Stockholm, Sweden. lennart.salmen@ri.se. 3. RISE Bioeconomy, Box 5604, 11486, Stockholm, Sweden. 4. Research Institute of Wood Industry of Chinese Academy of Forestry, Hunan Collaborative Innovation Center for Effective Utilizing of Wood and Bamboo Resources, Beijing, 100091, People's Republic of China. jianxiong@caf.ac.cn.
Abstract
MAIN CONCLUSION: Glucomannan was more strongly oriented, in line with the orientation of cellulose, than the xylan in both compression wood and normal wood of Chinese fir. Lignin in compression wood was somewhat more oriented in the direction of the cellulose microfibrils than in normal wood. The structural organization in compression wood (CW) is quite different from that in normal wood (NW). To shed more light on the structural organization of the polymers in plant cell walls, Fourier Transform Infrared (FTIR) microscopy in transmission mode has been used to compare the S2-dominated mean orientation of wood polymers in CW with that in NW from Chinese fir (Cunninghamia lanceolata). Polarized FTIR measurements revealed that in both CW and NW samples, glucomannan and xylan showed a parallel orientation with respect to the cellulose microfibrils. In both wood samples, the glucomannan showed a much greater degree of orientation than the xylan, indicating that the glucomannan has established a stronger interaction with cellulose than xylan. For the lignin, the absorption peak also indicated an orientation along the direction of the cellulose microfibrils, but this orientation was more pronounced in CW than in NW, indicating that the lignin is affected by the orientation of the cellulose microfibrils more strongly in CW than it is in NW.
MAIN CONCLUSION: Glucomannan was more strongly oriented, in line with the orientation of cellulose, than the xylan in both compression wood and normal wood of Chinese fir. Lignin in compression wood was somewhat more oriented in the direction of the cellulose microfibrils than in normal wood. The structural organization in compression wood (CW) is quite different from that in normal wood (NW). To shed more light on the structural organization of the polymers in plant cell walls, Fourier Transform Infrared (FTIR) microscopy in transmission mode has been used to compare the S2-dominated mean orientation of wood polymers in CW with that in NW from Chinese fir (Cunninghamia lanceolata). Polarized FTIR measurements revealed that in both CW and NW samples, glucomannan and xylan showed a parallel orientation with respect to the cellulose microfibrils. In both wood samples, the glucomannan showed a much greater degree of orientation than the xylan, indicating that the glucomannan has established a stronger interaction with cellulose than xylan. For the lignin, the absorption peak also indicated an orientation along the direction of the cellulose microfibrils, but this orientation was more pronounced in CW than in NW, indicating that the lignin is affected by the orientation of the cellulose microfibrils more strongly in CW than it is in NW.
Authors: Nicholas J Grantham; Joel Wurman-Rodrich; Oliver M Terrett; Jan J Lyczakowski; Katherine Stott; Dinu Iuga; Thomas J Simmons; Mylene Durand-Tardif; Steven P Brown; Ray Dupree; Marta Busse-Wicher; Paul Dupree Journal: Nat Plants Date: 2017-10-09 Impact factor: 15.793
Authors: Marta Busse-Wicher; Nicholas J Grantham; Jan J Lyczakowski; Nino Nikolovski; Paul Dupree Journal: Biochem Soc Trans Date: 2016-02 Impact factor: 5.407
Authors: Marta Busse-Wicher; Thiago C F Gomes; Theodora Tryfona; Nino Nikolovski; Katherine Stott; Nicholas J Grantham; David N Bolam; Munir S Skaf; Paul Dupree Journal: Plant J Date: 2014-07-15 Impact factor: 6.417