Eun Seon Yoo1, Gang Sik Choo1, Sung Hyun Kim1, Joong Seok Woo1, Hyeong Jin Kim1, Young Seok Park1, Byeong Soo Kim1, Sang Ki Kim1, Byung Kwon Park1, Sung Dae Cho2, Jeong Seok Nam3, Chang Sun Choi4, Jeong Hwan Che5,6, Ji-Youn Jung7. 1. Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea. 2. Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea. 3. Gwangju Institute of Science and Technology, School of Life Sciences, Gwangju, Republic of Korea. 4. School of Food Science and Technology, Chung-ang University, Ansung, Republic of Korea. 5. Biomedical Center for Animal Resource Development, Seoul National University College of Medicine, Seoul, Republic of Korea. 6. Biomedical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea. 7. Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea wangza@kongju.ac.kr.
Abstract
BACKGROUND/AIM: Piperine is a major pungent alkaloid present in black pepper (Piper nigrum L). This study investigated the potential anticancer effects of piperine on human melanoma cells and explored the potential pharmacological mechanisms in vitro and in vivo. MATERIALS AND METHODS: Studies were performed using the MTT assay, 4',6-diamidino-2-phenylindole (DAPI) staining, western blotting, a xenograft model, the terminal deoxynucleotidyl transferase dUTP nick end labeling assay, and immunohistochemistry. RESULTS: Piperine inhibited the growth of melanoma cells. Several apoptotic events were observed following treatment, as revealed by DAPI staining. Piperine increased the expression of BCL2-associated X, apoptosis regulator (BAX), cleaved poly(ADP-ribose)polymerase, cleaved caspase-9, phospho-c-Jun N-terminal kinase and phospho-p38, and reduced that of B-cell lymphoma 2 (BCL2), X-chromosome-linked inhibitor of apoptosis, and phospho-extracellular signal-regulated protein kinase (ERK1/2) in a concentration-dependent manner. Treatment of mice for 4 weeks with piperine inhibited tumor growth without apparent toxicity. Piperine increased the expression of apoptotic cells and cleaved-caspase-3 protein and reduced the expression of phospho-ERK1/2 protein in melanoma tumors. CONCLUSION: Piperine suppressed the growth of human melanoma cells by the induction of apoptosis via the inhibition of tumor growth of human melanoma cells and tumor xenograft models. Copyright
BACKGROUND/AIM: Piperine is a major pungent alkaloid present in black pepper (Piper nigrum L). This study investigated the potential anticancer effects of piperine on humanmelanoma cells and explored the potential pharmacological mechanisms in vitro and in vivo. MATERIALS AND METHODS: Studies were performed using the MTT assay, 4',6-diamidino-2-phenylindole (DAPI) staining, western blotting, a xenograft model, the terminal deoxynucleotidyl transferase dUTP nick end labeling assay, and immunohistochemistry. RESULTS:Piperine inhibited the growth of melanoma cells. Several apoptotic events were observed following treatment, as revealed by DAPI staining. Piperine increased the expression of BCL2-associated X, apoptosis regulator (BAX), cleaved poly(ADP-ribose)polymerase, cleaved caspase-9, phospho-c-Jun N-terminal kinase and phospho-p38, and reduced that of B-cell lymphoma 2 (BCL2), X-chromosome-linked inhibitor of apoptosis, and phospho-extracellular signal-regulated protein kinase (ERK1/2) in a concentration-dependent manner. Treatment of mice for 4 weeks with piperine inhibited tumor growth without apparent toxicity. Piperine increased the expression of apoptotic cells and cleaved-caspase-3 protein and reduced the expression of phospho-ERK1/2 protein in melanoma tumors. CONCLUSION:Piperine suppressed the growth of humanmelanoma cells by the induction of apoptosis via the inhibition of tumor growth of humanmelanoma cells and tumor xenograft models. Copyright
Authors: Julia Quarti; Daianne N M Torres; Erika Ferreira; Raphael S Vidal; Fabiana Casanova; Luciana B Chiarini; Eliane Fialho; Vivian M Rumjanek Journal: Molecules Date: 2021-02-10 Impact factor: 4.411
Authors: Khaled AbouAitah; Imane M Higazy; Anna Swiderska-Sroda; Reda M Abdelhameed; Stanislaw Gierlotka; Tarik A Mohamed; Urszula Szałaj; Witold Lojkowski Journal: Drug Deliv Date: 2021-12 Impact factor: 6.819
Authors: Khaled AbouAitah; Agata Stefanek; Iman M Higazy; Magdalena Janczewska; Anna Swiderska-Sroda; Agnieszka Chodara; Jacek Wojnarowicz; Urszula Szałaj; Samar A Shahein; Ahmed M Aboul-Enein; Faten Abou-Elella; Stanislaw Gierlotka; Tomasz Ciach; Witold Lojkowski Journal: Pharmaceutics Date: 2020-01-16 Impact factor: 6.321