Michal Mego1,2, Marian Karaba2,3, Gabriel Minarik4, Juraj Benca2,5, Jurisova Silvia6,2, Tatiana Sedlackova4, Denisa Manasova6,2, Katarina Kalavska6,2, Daniel Pindak2,3, Massimo Cristofanilli7, James M Reuben8, Jozef Mardiak6,2. 1. 2nd Department of Oncology, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic misomego@gmail.com. 2. National Cancer Institute, Bratislava, Slovak Republic. 3. Department of Surgical Oncology, Slovak Medical University, Bratislava, Slovak Republic. 4. Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic. 5. Department of Medicine, St. Elizabeth University, Bratislava, Slovak Republic. 6. 2nd Department of Oncology, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic. 7. Division of Hematology-Oncology at Northwestern University Feinberg School of Medicine, Northwestern University, Chicago, IL, U.S.A. 8. Department of Hematopathology, University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.
Abstract
BACKGROUND/AIM: Circulating tumor cells (CTCs) comprise a heterogeneous population of cancer cells with different clinical and biological value. The aim of this study was to evaluate the prognostic value of CTCs with an epithelial-mesenchymal transition (EMT) phenotype in primary breast cancer (PBC) patients. PATIENTS AND METHODS: This study included 427 primary breast cancer patients. RNA extracted from CD45-depleted peripheral blood mononuclear cell (PBMCs) was evaluated for the expression of EMT transcription factors (TWIST1, SNAIL1, SLUG, ZEB1) by quantitative real time polymerase chain reaction (qRT-PCR). RESULTS: In total, CTC EMT was detected in 77 (18.0%) patients. Patients without detectable CTC EMT in peripheral blood had significantly longer disease-free survival than patients with detectable CTC EMT. The prognostic value of CTC EMT was demonstrated in all subgroups of patients. CONCLUSION: CTCs with an EMT phenotype have a prognostic value in primary breast cancer. Copyright
BACKGROUND/AIM: Circulating tumor cells (CTCs) comprise a heterogeneous population of cancer cells with different clinical and biological value. The aim of this study was to evaluate the prognostic value of CTCs with an epithelial-mesenchymal transition (EMT) phenotype in primary breast cancer (PBC) patients. PATIENTS AND METHODS: This study included 427 primary breast cancerpatients. RNA extracted from CD45-depleted peripheral blood mononuclear cell (PBMCs) was evaluated for the expression of EMT transcription factors (TWIST1, SNAIL1, SLUG, ZEB1) by quantitative real time polymerase chain reaction (qRT-PCR). RESULTS: In total, CTC EMT was detected in 77 (18.0%) patients. Patients without detectable CTC EMT in peripheral blood had significantly longer disease-free survival than patients with detectable CTC EMT. The prognostic value of CTC EMT was demonstrated in all subgroups of patients. CONCLUSION: CTCs with an EMT phenotype have a prognostic value in primary breast cancer. Copyright
Authors: Michal Mego; Marian Karaba; Tatiana Sedlackova; Juraj Benca; Gabriela Repiska; Lucia Krasnicanova; Jan Macuch; Gabriela Sieberova; Silvia Jurisova; Daniel Pindak; Katarina Kalavska; Jozef Mardiak; Gabriel Minarik Journal: Mol Clin Oncol Date: 2020-04-01
Authors: Pavol Zubor; Peter Kubatka; Karol Kajo; Zuzana Dankova; Hubert Polacek; Tibor Bielik; Erik Kudela; Marek Samec; Alena Liskova; Dominika Vlcakova; Tatiana Kulkovska; Igor Stastny; Veronika Holubekova; Jan Bujnak; Zuzana Laucekova; Dietrich Büsselberg; Mariusz Adamek; Walther Kuhn; Jan Danko; Olga Golubnitschaja Journal: Int J Mol Sci Date: 2019-06-13 Impact factor: 5.923
Authors: Maxim E Menyailo; Ustinia A Bokova; Elena E Ivanyuk; Anna A Khozyainova; Evgeny V Denisov Journal: Mol Diagn Ther Date: 2021-07-21 Impact factor: 4.074
Authors: Michal Mego; Katarina Kalavska; Marian Karaba; Gabriel Minarik; Juraj Benca; Tatiana Sedlackova; Paulina Gronesova; Dana Cholujova; Daniel Pindak; Jozef Mardiak; Peter Celec Journal: Cancers (Basel) Date: 2020-09-10 Impact factor: 6.639
Authors: Evan N Cohen; Gitanjali Jayachandran; Max R Hardy; Ananya M Venkata Subramanian; Xiangtian Meng; James M Reuben Journal: PLoS One Date: 2020-10-23 Impact factor: 3.240