Marium Khaleque1, Selina Akter2, Humaira Akhter3, Sirajul Islam Khan4, Anowara Begum5. 1. University of Dhaka, Dhaka, Bangladesh. marium.khq@gmail.com. 2. Jessore University of Science and Technology, Jessore, Bangladesh. s.akter@just.edu.bd. 3. University of Dhaka, Dhaka, Bangladesh. humaira@du.ac.bd. 4. University of Dhaka, Dhaka, Bangladesh. sikhan@du.ac.bd. 5. University of Dhaka, Dhaka, Bangladesh. anowara@du.ac.bd.
Abstract
INTRODUCTION: Urinary tract infection is the most frequently diagnosed kidney and urologic disease. METHODOLOGY: Whether the Escherichia coli strains responsible for urinary tract infection (UPEC) carry virulence properties of diarrheagenic E. coli (DEC), 56 UPEC strains were examined for the presence of DEC and UPEC characteristics (e.g. biofilm formation, hemolysis activity, virulence genes). RESULTS: Among 56 UPEC strains, 21 showed capable of biofilm formation and only 5 showed hemolysis activity on sheep blood agar. In Multiplex PCR on assessment of virulence genes related to uropathogenesis; 42% was found positive for papC gene, 27% was fim1 positive, 11% was afa positive and none was found positive for sfa. Most of the isolates were found carrying none of eight diarrhea associated genes (e.g. estA, eltB, vt1, vt2, eaeA, ea, ial and bfpA) as expected. Only seven isolates were found to harbor these genes: five genes i.e., vt2, ial, eltB, bfpA and ea were found in five different isolates and two isolates were positive for estA, among these two, one was found positive for fim1, papC along with estA, a UPEC strain containing virulent gene of ETEC strain. One isolate was found carrying fim1 and vt2 showing the property of EHEC and another isolate was found positive for fim1 and ial, the characteristic of EIEC. One isolate harboring bfpA gene characterized as EPEC and the another one was found to harbor ea gene, characterized as EAEC. CONCLUSIONS: This study observed that most UPEC strains are unique to uropathogenesis, still very few may carry the diarrheagenic property. Copyright (c) 2017 Marium Khaleque, Selina Akter, Humaira Akhter, Sirajul Islam Khan, Anowara Begum.
INTRODUCTION:Urinary tract infection is the most frequently diagnosed kidney and urologic disease. METHODOLOGY: Whether the Escherichia coli strains responsible for urinary tract infection (UPEC) carry virulence properties of diarrheagenic E. coli (DEC), 56 UPEC strains were examined for the presence of DEC and UPEC characteristics (e.g. biofilm formation, hemolysis activity, virulence genes). RESULTS: Among 56 UPEC strains, 21 showed capable of biofilm formation and only 5 showed hemolysis activity on sheep blood agar. In Multiplex PCR on assessment of virulence genes related to uropathogenesis; 42% was found positive for papC gene, 27% was fim1 positive, 11% was afa positive and none was found positive for sfa. Most of the isolates were found carrying none of eight diarrhea associated genes (e.g. estA, eltB, vt1, vt2, eaeA, ea, ial and bfpA) as expected. Only seven isolates were found to harbor these genes: five genes i.e., vt2, ial, eltB, bfpA and ea were found in five different isolates and two isolates were positive for estA, among these two, one was found positive for fim1, papC along with estA, a UPEC strain containing virulent gene of ETEC strain. One isolate was found carrying fim1 and vt2 showing the property of EHEC and another isolate was found positive for fim1 and ial, the characteristic of EIEC. One isolate harboring bfpA gene characterized as EPEC and the another one was found to harbor ea gene, characterized as EAEC. CONCLUSIONS: This study observed that most UPEC strains are unique to uropathogenesis, still very few may carry the diarrheagenic property. Copyright (c) 2017 Marium Khaleque, Selina Akter, Humaira Akhter, Sirajul Islam Khan, Anowara Begum.
Entities:
Keywords:
Diarrheagenic E. coli; ETEC; UTI; Uropathogenic E. coli; virulence genes
Authors: Hanif Ullah; Kashif Bashir; Muhammad Idrees; Amin Ullah; Neelma Hassan; Sara Khan; Bilal Nasir; Tariq Nadeem; Hina Ahsan; Muhammad Islam Khan; Qurban Ali; Sher Muhammad; Muhammad Afzal Journal: PLoS One Date: 2022-01-28 Impact factor: 3.240