Maryam Ahmadi 1 , Farshid Fathi 1 , Saloomeh Fouladi 1 , Fereshteh Alsahebfosul 1 , Mostafa Manian 2 , Nahid Eskandari 1,3 Show Affiliations »
Abstract
BACKGROUND: Asthma is a chronic and complex inflammatory disease of the respiratory tract. Also, multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system. Against this background, IL-33 and IL1RL1 play a critical role in autoimmune and inflammatory disorders. Here, we explored the IL-33 serum level and two potential genetic variants in the IL33 gene and its receptor in Iranian asthma and MS patients. METHODS: This study consisted of asthma (n=140) and MS patients (n=140), and healthy subjects (n=72). Genotyping was carried out in two genetic polymorphisms, rs1342326 variant of IL-33 and rs10204137SNP variant of IL-33 receptor genes, using High- Resolution Melt Real- Time PCR based method. The level of serum IL-33 was also measured using enzyme-linked immunosorbent assay method. RESULTS: The level of IL33 was significantly higher in asthma and MS patients compared to the control group (P< 0.001- P<0.001).The frequency distribution of the genotype in rs1342326 variant of IL-33 gene in patients with asthma, MS and healthy subjects was not significantly different (P>0.05). The frequency distribution of the genotype in rs10204137 variant of IL-33 gene in MS patients and healthy subjects was significantly different (p = 0.013). CONCLUSION: Our findings demonstrated that asthma and MS patients had a higher level of IL-33, and IL-33 receptor genetic polymorphism was associated with MS. Further studies in a larger multicenter setting are needed to explore the value of this marker as a risk stratification biomarker. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
BACKGROUND: Asthma is a chronic and complex inflammatory disease of the respiratory tract. Also, multiple sclerosis (MS ) is a chronic inflammatory demyelinating disease of the central nervous system . Against this background, IL-33 and IL1RL1 play a critical role in autoimmune and inflammatory disorders . Here, we explored the IL-33 serum level and two potential genetic variants in the IL33 gene and its receptor in Iranian asthma and MS patients . METHODS: This study consisted of asthma (n=140) and MS patients (n=140), and healthy subjects (n=72). Genotyping was carried out in two genetic polymorphisms, rs1342326 variant of IL-33 and rs10204137SNP variant of IL-33 receptor genes, using High- Resolution Melt Real- Time PCR based method. The level of serum IL-33 was also measured using enzyme-linked immunosorbent assay method. RESULTS: The level of IL33 was significantly higher in asthma and MS patients compared to the control group (P< 0.001- P<0.001).The frequency distribution of the genotype in rs1342326 variant of IL-33 gene in patients with asthma , MS and healthy subjects was not significantly different (P>0.05). The frequency distribution of the genotype in rs10204137 variant of IL-33 gene in MS patients and healthy subjects was significantly different (p = 0.013). CONCLUSION: Our findings demonstrated that asthma and MS patients had a higher level of IL-33 , and IL-33 receptor genetic polymorphism was associated with MS . Further studies in a larger multicenter setting are needed to explore the value of this marker as a risk stratification biomarker. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
Entities: Disease
Gene
Mutation
Species
Keywords:
Asthma; IL-33; IL-33 serum level; IL1RL1; Multiple Sclerosis; polymorphism.
Year: 2019
PMID： 30950351 DOI： 10.2174/1566524019666190405120137
Source DB: PubMed Journal: Curr Mol Med ISSN： 1566-5240 Impact factor: 2.222