Beili Wang1, Jie Zhu1, Xiaolu Ma1, Hao Wang1, Shuangjian Qiu2, Baishen Pan1, Jian Zhou2, Jia Fan2, Xinrong Yang2, Wei Guo3, Yunfeng Cheng4. 1. Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, 136 Yi Xue Yuan Road, Shanghai 200032, PR China. 2. Department of Liver Surgery, Liver Cancer Institute, Zhongshan Hospital, Fudan University, 136 Yi Xue Yuan Road, Shanghai 200032, PR China. 3. Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, 136 Yi Xue Yuan Road, Shanghai 200032, PR China. Electronic address: guo.wei@zs-hospital.sh.cn. 4. Department of Hematology, Zhongshan Hospital, Fudan University, 136 Yi Xue Yuan Road, Shanghai 200032, PR China. Electronic address: cheng.yunfeng@zs-hospital.sh.cn.
Abstract
BACKGROUND: The venous thromboembolism, which may be caused by increased platelet activation, is a risk factor for tumor prognosis. We determined the platelet activation status for diagnosis and predicting postoperative prognosis of hepatocellular carcinoma. METHODS: We conducted a prospective study of 191 patients diagnosed with HCC at Zhongshan Hospital from April 2016 to July 2016 as well as 99 healthy people. The platelet activation status was assessed by 2 platelet markers, PAC-1 and CD62p, using flow cytometry. The patients were treated with TACE or resection and monitored for ≥6 months. The diagnostic value of marker-positive platelets was determined by the receiver operating characteristic curve and the postoperative value were analyzed using the Kaplan-Meier method and COX regression model. RESULTS: All the 3 groups with high levels of marker-positive platelets were likely to be diagnosed with HCC and the PAC-1+ percentage had the best efficacy. The univariate analysis showed that the levels of PAC-1+ and CD62p+ platelets was risker factors for poor postoperative prognosis after both TACE and resection. Moreover, the multivariate analysis revealed that the level of PAC-1+ platelets was an independent risk factor for poor prognosis. CONCLUSIONS: The PAC-1+ percentage of platelets is a new indicator for diagnosis and predicting postoperative prognosis.
BACKGROUND: The venous thromboembolism, which may be caused by increased platelet activation, is a risk factor for tumor prognosis. We determined the platelet activation status for diagnosis and predicting postoperative prognosis of hepatocellular carcinoma. METHODS: We conducted a prospective study of 191 patients diagnosed with HCC at Zhongshan Hospital from April 2016 to July 2016 as well as 99 healthy people. The platelet activation status was assessed by 2 platelet markers, PAC-1 and CD62p, using flow cytometry. The patients were treated with TACE or resection and monitored for ≥6 months. The diagnostic value of marker-positive platelets was determined by the receiver operating characteristic curve and the postoperative value were analyzed using the Kaplan-Meier method and COX regression model. RESULTS: All the 3 groups with high levels of marker-positive platelets were likely to be diagnosed with HCC and the PAC-1+ percentage had the best efficacy. The univariate analysis showed that the levels of PAC-1+ and CD62p+ platelets was risker factors for poor postoperative prognosis after both TACE and resection. Moreover, the multivariate analysis revealed that the level of PAC-1+ platelets was an independent risk factor for poor prognosis. CONCLUSIONS: The PAC-1+ percentage of platelets is a new indicator for diagnosis and predicting postoperative prognosis.
Authors: Marion Mussbacher; Laura Brunnthaler; Anja Panhuber; Patrick Starlinger; Alice Assinger Journal: Int J Mol Sci Date: 2021-03-18 Impact factor: 5.923