Detao Ding1, Zhiling Qi. 1. Department of Otolaryngology, Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, P.R.China.
Abstract
PURPOSE: This study aimed to investigate the expression pattern of microRNA (miRNA)-195 in laryngeal carcinoma and analyze the relationships of miRNA-195 expression with clinicopathological features and prognosis. METHODS: The expression levels of miRNA-195 in pathological cancer tissue and tumor-adjacent normal tissue of 182 patients with laryngeal carcinoma were measured by reverse transcription-polymerase chain reaction (RT-PCR), and the relationships of relative expression levels of miRNA-195 with clinicopathological features and prognosis of patients with laryngeal carcinoma were analyzed. The invasive ability of cells was measured by Transwell chamber test. Cell proliferation in vitro was determined using 3-(4,5)-dimethylthiahiazo-(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) method. RESULTS: The expression level of miRNA-195 in laryngeal carcinoma tissue was lower than that in tumor-adjacent normal tissue (p<0.05). In vitro growth, migration and invasion ability of laryngeal carcinoma cells with lower expression level of miRNA-195 were distinctly higher than those of normal tissue cells. The expression levels of miRNA-195 were not significantly correlated with gender, age, grade of differentiation and tumor site (p>0.05), but were closely correlated with lymph node metastasis and clinical staging (p<0.05). The 5-year survival rate, median survival and progression-free survival in patients with high expression levels of miRNA-195 were significantly better than those in patients with low expression levels of miRNA-195 (78 vs. 39%, 57 months vs. 39 months, 40 months vs. 27 months, p<0.05). CONCLUSION: These findings indicated that expression of miRNA-195 in laryngeal carcinoma tissue is down-regulated, and the low expression of miRNA-195 may be related to invasion and metastasis of laryngeal carcinoma, which indicates poor prognosis of patients. MiRNA-195 may serve as a potential molecular target for the treatment and prognosis evaluation of laryngeal carcinoma.
PURPOSE: This study aimed to investigate the expression pattern of microRNA (miRNA)-195 in laryngeal carcinoma and analyze the relationships of miRNA-195 expression with clinicopathological features and prognosis. METHODS: The expression levels of miRNA-195 in pathological cancer tissue and tumor-adjacent normal tissue of 182 patients with laryngeal carcinoma were measured by reverse transcription-polymerase chain reaction (RT-PCR), and the relationships of relative expression levels of miRNA-195 with clinicopathological features and prognosis of patients with laryngeal carcinoma were analyzed. The invasive ability of cells was measured by Transwell chamber test. Cell proliferation in vitro was determined using 3-(4,5)-dimethylthiahiazo-(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) method. RESULTS: The expression level of miRNA-195 in laryngeal carcinoma tissue was lower than that in tumor-adjacent normal tissue (p<0.05). In vitro growth, migration and invasion ability of laryngeal carcinoma cells with lower expression level of miRNA-195 were distinctly higher than those of normal tissue cells. The expression levels of miRNA-195 were not significantly correlated with gender, age, grade of differentiation and tumor site (p>0.05), but were closely correlated with lymph node metastasis and clinical staging (p<0.05). The 5-year survival rate, median survival and progression-free survival in patients with high expression levels of miRNA-195 were significantly better than those in patients with low expression levels of miRNA-195 (78 vs. 39%, 57 months vs. 39 months, 40 months vs. 27 months, p<0.05). CONCLUSION: These findings indicated that expression of miRNA-195 in laryngeal carcinoma tissue is down-regulated, and the low expression of miRNA-195 may be related to invasion and metastasis of laryngeal carcinoma, which indicates poor prognosis of patients. MiRNA-195 may serve as a potential molecular target for the treatment and prognosis evaluation of laryngeal carcinoma.