Jinyong Zhang1,2,3, Muchun He4, Zilong Xiang5, Shufang Liu6,7,8, ZhiMeng Zhuang9,10. 1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China. 2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China. 3. Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266200, China. 4. Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China. 5. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China. 6. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China. liusf@ysfri.ac.cn. 7. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China. liusf@ysfri.ac.cn. 8. Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266200, China. liusf@ysfri.ac.cn. 9. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China. zhuangzm@ysfri.ac.cn. 10. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China. zhuangzm@ysfri.ac.cn.
Abstract
BACKGROUND: The subpeduncle lobe/olfactory lobe-optic gland axis is called the endocrine regulation center of cephalopods. However, little is known about the mechanism of the subpeduncle lobe/olfactory lobe-optic gland axis regulate the sexual maturation and post-reproductive death of Sepia esculenta Hoyle. OBJECTIVES: The primary objective of this study was to provide basic information for revealing the mechanism of the subpeduncle lobe/olfactory lobe-optic axis regulating the rapid post-reproductive death of S. esculenta. METHODS: In this paper, Illumina sequencing based transcriptome analysis was performed on the brain tissue of female S. esculenta in the three key developmental stages: growth stage (BG), spawning stage (BS), and post-reproductive death stage (BA). RESULTS: A total of 66.19 Gb Illumina sequencing data were obtained. A comparative analysis of the three stages showed 2609, 3333, and 170 differentially expressed genes (DEGs) in BG-vs-BA, BG-vs-BA, and BS-vs-BA, respectively. The Gene Ontology (GO) enrichment analysis of DEGs revealed that the regulation of cyclin-dependent protein serine/threonine kinase activity, oxidative phosphorylation, and respiratory chain were significantly enriched. The significant enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway identified pathways associated with the regulation of death, such as the mammalian target of rapamycin (mTOR) signaling pathway, AMPK signaling pathway, oxidative phosphorylation, and cell cycle. CONCLUSION: The post-reproductive death of S. esculenta was found to be a complex energy steady-state regulation network system. The mTOR acted as an energy receptor and had a key role in regulating energy homeostasis.
BACKGROUND: The subpeduncle lobe/olfactory lobe-optic gland axis is called the endocrine regulation center of cephalopods. However, little is known about the mechanism of the subpeduncle lobe/olfactory lobe-optic gland axis regulate the sexual maturation and post-reproductive death of Sepia esculenta Hoyle. OBJECTIVES: The primary objective of this study was to provide basic information for revealing the mechanism of the subpeduncle lobe/olfactory lobe-optic axis regulating the rapid post-reproductive death of S. esculenta. METHODS: In this paper, Illumina sequencing based transcriptome analysis was performed on the brain tissue of female S. esculenta in the three key developmental stages: growth stage (BG), spawning stage (BS), and post-reproductive death stage (BA). RESULTS: A total of 66.19 Gb Illumina sequencing data were obtained. A comparative analysis of the three stages showed 2609, 3333, and 170 differentially expressed genes (DEGs) in BG-vs-BA, BG-vs-BA, and BS-vs-BA, respectively. The Gene Ontology (GO) enrichment analysis of DEGs revealed that the regulation of cyclin-dependent protein serine/threonine kinase activity, oxidative phosphorylation, and respiratory chain were significantly enriched. The significant enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway identified pathways associated with the regulation of death, such as the mammalian target of rapamycin (mTOR) signaling pathway, AMPK signaling pathway, oxidative phosphorylation, and cell cycle. CONCLUSION: The post-reproductive death of S. esculenta was found to be a complex energy steady-state regulation network system. The mTOR acted as an energy receptor and had a key role in regulating energy homeostasis.
Authors: Owen R Jones; Alexander Scheuerlein; Roberto Salguero-Gómez; Carlo Giovanni Camarda; Ralf Schaible; Brenda B Casper; Johan P Dahlgren; Johan Ehrlén; María B García; Eric S Menges; Pedro F Quintana-Ascencio; Hal Caswell; Annette Baudisch; James W Vaupel Journal: Nature Date: 2013-12-08 Impact factor: 49.962