BACKGROUND: Bacteria and bacterial components have been associated with the activation of coagulation factors and initiating the blood clot formation. The aim of this study was to investigate whether bacterial populations are present in clots retrieved from patients that have suffered a large vessel occlusion acute ischemic stroke (AIS). MATERIALS AND METHODS: Clot samples were collected from 20 AIS patients who underwent clot retrieval with mechanical thrombectomy. Patient clinical demographic details were noted. Expression of bacterial 16S rDNA was analyzed by standard and real-time polymerase chain reaction (PCR). Gram staining was performed to identify Gram-positive and Gram-negative bacteria. RESULTS: Both the real-time and standard PCR demonstrated no expression of 16S rDNA in any of the 20 clots samples from AIS patients. Gram staining results showed no expression of Gram-positive or Gram-negative bacteria present in the clot samples. CONCLUSION: Our current study found no bacteria populations in the clots of AIS patients.
BACKGROUND: Bacteria and bacterial components have been associated with the activation of coagulation factors and initiating the blood clot formation. The aim of this study was to investigate whether bacterial populations are present in clots retrieved from patients that have suffered a large vessel occlusion acute ischemic stroke (AIS). MATERIALS AND METHODS: Clot samples were collected from 20 AISpatients who underwent clot retrieval with mechanical thrombectomy. Patient clinical demographic details were noted. Expression of bacterial 16S rDNA was analyzed by standard and real-time polymerase chain reaction (PCR). Gram staining was performed to identify Gram-positive and Gram-negative bacteria. RESULTS: Both the real-time and standard PCR demonstrated no expression of 16S rDNA in any of the 20 clots samples from AISpatients. Gram staining results showed no expression of Gram-positive or Gram-negative bacteria present in the clot samples. CONCLUSION: Our current study found no bacteria populations in the clots of AISpatients.
Authors: Tanja Pessi; Vesa Karhunen; Pasi P Karjalainen; Antti Ylitalo; Juhani K Airaksinen; Matti Niemi; Mikko Pietila; Kari Lounatmaa; Teppo Haapaniemi; Terho Lehtimäki; Reijo Laaksonen; Pekka J Karhunen; Jussi Mikkelsson Journal: Circulation Date: 2013-02-15 Impact factor: 29.690
Authors: Mitchell S V Elkind; Pankajavalli Ramakrishnan; Yeseon P Moon; Bernadette Boden-Albala; Khin M Liu; Steve L Spitalnik; Tanja Rundek; Ralph L Sacco; Myunghee C Paik Journal: Arch Neurol Date: 2009-11-09