Pei-Chi Chen1, Yi-Wen Chien2, Suh-Ching Yang3. 1. Division of Endocrinology and Metabolism, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan; School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan. 2. School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan; Research Center of Geriatric Nutrition, College of Nutrition, Taipei Medical University, Taipei, Taiwan. 3. School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan; Research Center of Geriatric Nutrition, College of Nutrition, Taipei Medical University, Taipei, Taiwan; Nutrition Research Center, Taipei Medical University Hospital, Taipei, Taiwan. Electronic address: sokei@tmu.edu.tw.
Abstract
OBJECTIVES: Gut microbiota dysbiosis is known to be associated with diabetes; however, the findings of previous studies are conflicting. To clarify the association between type 2 diabetes and the gut microbiota, the present study analyzed the composition of fecal gut microbiota and its correlation with specific clinical parameters in newly diagnosed, treatment-naive diabetic patients and healthy controls. METHODS: A total of 50 patients with newly diagnosed type 2 diabetes and 50 healthy control participants were enrolled in the study. Fecal samples, blood samples, and food diaries were collected from the diabetic patients before and 3 mo after the start of their antidiabetic treatment. These samples were also collected from the healthy controls. The gut microbiota was characterized by 16S ribosomal RNA analysis using quantitative polymerase chain reaction. RESULTS: The fecal count of Lactobacillus was significantly higher, whereas Clostridium coccoides and Clostridium leptum were significantly lower in the diabetic patients compared with the healthy controls. Lactobacillus was significantly positively correlated with glucose, glycated hemoglobin, and the homeostatic model assessment, whereas C. coccoides and C. leptum were significantly negatively correlated with the diabetic parameters. In addition, the newly diagnosed diabetic patients had a significant decrease in the presence of C. coccoides and C. leptum after 3 mo of treatment compared with before treatment. CONCLUSIONS: The amount of fecal Lactobacillus, C. coccoides, and C. leptum was significantly different between the patients with type 2 diabetes and the healthy controls. The levels of Clostridium were also significantly changed after 3 mo of treatment in the diabetic patients. Further research is needed to clarify the correlation or causal relationship between the gut microbiota dysbiosis and type 2 diabetes.
OBJECTIVES: Gut microbiota dysbiosis is known to be associated with diabetes; however, the findings of previous studies are conflicting. To clarify the association between type 2 diabetes and the gut microbiota, the present study analyzed the composition of fecal gut microbiota and its correlation with specific clinical parameters in newly diagnosed, treatment-naive diabeticpatients and healthy controls. METHODS: A total of 50 patients with newly diagnosed type 2 diabetes and 50 healthy control participants were enrolled in the study. Fecal samples, blood samples, and food diaries were collected from the diabeticpatients before and 3 mo after the start of their antidiabetic treatment. These samples were also collected from the healthy controls. The gut microbiota was characterized by 16S ribosomal RNA analysis using quantitative polymerase chain reaction. RESULTS: The fecal count of Lactobacillus was significantly higher, whereas Clostridium coccoides and Clostridium leptum were significantly lower in the diabeticpatients compared with the healthy controls. Lactobacillus was significantly positively correlated with glucose, glycated hemoglobin, and the homeostatic model assessment, whereas C. coccoides and C. leptum were significantly negatively correlated with the diabetic parameters. In addition, the newly diagnosed diabeticpatients had a significant decrease in the presence of C. coccoides and C. leptum after 3 mo of treatment compared with before treatment. CONCLUSIONS: The amount of fecal Lactobacillus, C. coccoides, and C. leptum was significantly different between the patients with type 2 diabetes and the healthy controls. The levels of Clostridium were also significantly changed after 3 mo of treatment in the diabeticpatients. Further research is needed to clarify the correlation or causal relationship between the gut microbiota dysbiosis and type 2 diabetes.
Authors: Maria Jose Carranza-Naval; Maria Vargas-Soria; Carmen Hierro-Bujalance; Gloria Baena-Nieto; Monica Garcia-Alloza; Carmen Infante-Garcia; Angel Del Marco Journal: Biomolecules Date: 2021-02-10