Extended depth-of-field all-optical photoacoustic microscopy with a dual non-diffracting Bessel beam.

All-optical photoacoustic microscopy (AOPAM) facilitates high-sensitivity, wide-bandwidth, volumetric imaging without coupling media. However, the rapid divergence of the Gaussian beam restricts the stability and depth-of-field in typical Gaussian AOPAM (G-AOPAM). Here we report an extended depth-of-field AOPAM using a dual non-diffracting Bessel beam (B-AOPAM). Benefiting from the designing, the B-AOPAM has the unique advantages of increasing depth resolving ability and improving photoacoustic detection sensitivity. The proposed scheme shows optimal lateral resolution of 2.4 μm and a long depth-of-focus of 635 μm, which is 10-fold larger than that of the G-AOPAM. The scattering phantoms and in vivo animal experiments demonstrated the imaging feasibility and capability of the B-AOPAM, which can provide noncontact, high spatial resolution imaging of non-flat tissue and contribute to future clinical applications.