| Literature DB >> 3092784 |
D D Pekovic, J P Chausseau, N Lapointe, J Michaud, S Garzon, H Strykowski, C Tsoukas, N Gilmore, H Goldman, M Gornitsky.
Abstract
HTLV-III was searched for in frozen sections of peripheral blood lymphocytes obtained from AIDS patients by an immunofluorescence technique. Human IgG against HTLV-III/LAV and monoclonal antibodies against HTLV-III/LAV P 24 antigen, yielded a strong cytoplasmic fluorescence in frozen sections of the lymphocytes. Some cells containing HTLV-III antigens displayed multinucleated giant forms. They also reacted with monoclonal antibodies against helper/inducer T-cells (OKT4+), as demonstrated by direct double staining immunofluorescence. Similarly, complexes of immunoglobulins and C3 component of complement were also detected on HTLV-III/LAV Ag expressing lymphocytes. Immunofluorescence study of frozen sections of peripheral blood lymphocytes appeared to be a simple, fast and reliable method for detection of HTLV-III/LAV Ag in AIDS patients.Entities:
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Year: 1986 PMID： 3092784 DOI： 10.1007/bf01316724
Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574