Ching-Yan Chloé Yeung1,2, Peter Schjerling3,4, Katja M Heinemeier3,4, Anders P Boesen3,4, Kasper Dideriksen3,4, Michael Kjær3,4. 1. Institute of Sports Medicine Copenhagen, Bispebjerg Hospital, Building 8, Nielsine Nielsens Vej 11, 2400, Copenhagen NV, Denmark. chloe.yeung@gmail.com. 2. Center for Healthy Aging, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen N, Denmark. chloe.yeung@gmail.com. 3. Institute of Sports Medicine Copenhagen, Bispebjerg Hospital, Building 8, Nielsine Nielsens Vej 11, 2400, Copenhagen NV, Denmark. 4. Center for Healthy Aging, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen N, Denmark.
Abstract
PURPOSE: The discovery of musculoskeletal tissues, including muscle, tendons, and cartilage, as peripheral circadian clocks strongly implicates their role in tissue-specific homeostasis. Age-related dampening and misalignment of the tendon circadian rhythm and its outputs may be responsible for the decline in tendon homeostasis. It is unknown which entrainment signals are responsible for the synchronization of the tendon clock to the light-dark cycle. METHODS: We sought to examine any changes in the expression levels of core clock genes (BMAL1, CLOCK, PER2, CRY1, and NR1D1) in healthy human patellar tendon biopsies obtained from three different intervention studies: increased physical activity (leg kicksfor 1 h) in young, reduced activity (2 weeks immobilization of one leg) in young, and in old tendons. RESULTS: The expression level of clock genes in human tendon in vivo was very low and a high variation between individuals was found. We were thus unable to detect any differences in core clock gene expression neither after acute exercise nor immobilization. CONCLUSIONS: We are unable to find evidence for an effect of exercise or immobilization on circadian clock gene expression in human tendon samples.
RCT Entities:
PURPOSE: The discovery of musculoskeletal tissues, including muscle, tendons, and cartilage, as peripheral circadian clocks strongly implicates their role in tissue-specific homeostasis. Age-related dampening and misalignment of the tendon circadian rhythm and its outputs may be responsible for the decline in tendon homeostasis. It is unknown which entrainment signals are responsible for the synchronization of the tendon clock to the light-dark cycle. METHODS: We sought to examine any changes in the expression levels of core clock genes (BMAL1, CLOCK, PER2, CRY1, and NR1D1) in healthy human patellar tendon biopsies obtained from three different intervention studies: increased physical activity (leg kicks for 1 h) in young, reduced activity (2 weeks immobilization of one leg) in young, and in old tendons. RESULTS: The expression level of clock genes in human tendon in vivo was very low and a high variation between individuals was found. We were thus unable to detect any differences in core clock gene expression neither after acute exercise nor immobilization. CONCLUSIONS: We are unable to find evidence for an effect of exercise or immobilization on circadian clock gene expression in human tendon samples.
Authors: Gang Wu; Marc D Ruben; Robert E Schmidt; Lauren J Francey; David F Smith; Ron C Anafi; Jacob J Hughey; Ryan Tasseff; Joseph D Sherrill; John E Oblong; Kevin J Mills; John B Hogenesch Journal: Proc Natl Acad Sci U S A Date: 2018-10-30 Impact factor: 11.205
Authors: J Farup; S K Rahbek; M H Vendelbo; A Matzon; J Hindhede; A Bejder; S Ringgard; K Vissing Journal: Scand J Med Sci Sports Date: 2013-05-07 Impact factor: 4.221
Authors: Benjamin F Miller; Jens L Olesen; Mette Hansen; Simon Døssing; Regina M Crameri; Rasmus J Welling; Henning Langberg; Allan Flyvbjerg; Michael Kjaer; John A Babraj; Kenneth Smith; Michael J Rennie Journal: J Physiol Date: 2005-07-07 Impact factor: 5.182
Authors: Rok Kosir; Jure Acimovic; Marko Golicnik; Martina Perse; Gregor Majdic; Martina Fink; Damjana Rozman Journal: BMC Mol Biol Date: 2010-08-16 Impact factor: 2.946
Authors: Simon Doessing; Katja M Heinemeier; Lars Holm; Abigail L Mackey; Peter Schjerling; Michael Rennie; Kenneth Smith; Søren Reitelseder; Anne-Marie Kappelgaard; Michael Højby Rasmussen; Allan Flyvbjerg; Michael Kjaer Journal: J Physiol Date: 2009-11-23 Impact factor: 5.182
Authors: John J McCarthy; Jessica L Andrews; Erin L McDearmon; Kenneth S Campbell; Brigham K Barber; Brooke H Miller; John R Walker; John B Hogenesch; Joseph S Takahashi; Karyn A Esser Journal: Physiol Genomics Date: 2007-06-05 Impact factor: 3.107
Authors: David Merinero; Manuel Rodríguez-Aragón; Javier Álvarez-González; Álvaro López-Samanes; Joaquín López-Pascual Journal: Int J Environ Res Public Health Date: 2021-01-15 Impact factor: 3.390
Authors: Finn Johannsen; Jens Lykkegaard Olesen; Tommy Frisgaard Øhlenschläger; Mathilde Lundgaard-Nielsen; Camilla Kjaer Cullum; Anna Svarre Jakobsen; Michael Skovdal Rathleff; Peter Stig Magnusson; Michael Kjær Journal: JAMA Netw Open Date: 2022-07-01
Authors: Cristina Camello-Almaraz; Francisco E Martin-Cano; Francisco J Santos; Mª Teresa Espin; Juan Antonio Madrid; Maria J Pozo; Pedro J Camello Journal: Int J Mol Sci Date: 2020-01-20 Impact factor: 5.923