Linn Salto Mamsen1, Karol Charkiewicz2, Richard A Anderson3, Evelyn E Telfer4, Marie McLaughlin4, Thomas W Kelsey5, Stine G Kristensen6, Debra A Gook7, Erik Ernst8, Claus Yding Andersen6. 1. Laboratory of Reproductive Biology, The Juliane Marie Centre for Women, Children and Reproduction, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark. Electronic address: linn.salto.mamsen@regionh.dk. 2. Laboratory of Reproductive Biology, The Juliane Marie Centre for Women, Children and Reproduction, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark; Department of Perinatology and Obstetrics, Medical University of Bialystok, Bialystok, Poland. 3. Medical Research Council Centre for Reproductive Health, University of Edinburgh, Edinburgh, United Kingdom. 4. Institute of Cell Biology, School of Biological Sciences and Genes and Development Group, School of Biomedical Sciences, University of Edinburgh, Edinburgh, United Kingdom. 5. University of St. Andrews, School of Computer Science, North Haugh, St. Andrews, United Kingdom. 6. Laboratory of Reproductive Biology, The Juliane Marie Centre for Women, Children and Reproduction, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark. 7. Reproductive Services and Melbourne IVF, Royal Women's Hospital, Parkville, Victoria, Australia; Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, Victoria, Australia. 8. Department of Obstetrics and Gynaecology, Randers Regional Hospital, Randers, Denmark.
Abstract
OBJECTIVE: To characterize ovarian follicles of girls and young women with Turner syndrome (TS) who underwent ovarian tissue cryopreservation (OTC). DESIGN: Retrospective case-control study. SETTING: University hospital. PATIENT(S): Fifteen girls and young women with TS aged 5-22 years at OTC were included, together with 42 control girls and young women aged 1-25 years who underwent OTC because of cancer. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Follicle density (follicles/mm3), morphology, and health were assessed in ovarian cortex biopsies from TS patients and compared with controls. Hormone concentrations were measured in serum and follicle fluids. Immature cumulus oocyte complexes were obtained and matured in vitro. RESULT(S): Follicles were found in 60% of the biopsies (9 of 15) from TS ovaries. In 78% of the ovaries (7 of 9) with follicles, the follicle density was within the 95% confidence interval of the control group. There was a high rate of abnormal follicle morphology. Six follicle-specific proteins were expressed similarly in TS and control ovaries. However, apoptosis and zona pellucida protein expression were found to be abnormal in TS. Turner syndrome follicle fluid from small antral follicles had lower concentrations of estrogen and testosterone and higher concentrations of antimüllerian hormone than controls. Thirty-one cumulus oocyte complexes were collected from one patient and cultured for 48 hours in vitro, resulting in five metaphase II oocytes (maturation rate 16%, degeneration rate 19%). CONCLUSION(S): The benefits of OTC may be limited to a highly selected group of TS mosaic patients in whom a sizeable pool of normal follicles is present at OTC.
OBJECTIVE: To characterize ovarian follicles of girls and young women with Turner syndrome (TS) who underwent ovarian tissue cryopreservation (OTC). DESIGN: Retrospective case-control study. SETTING: University hospital. PATIENT(S): Fifteen girls and young women with TS aged 5-22 years at OTC were included, together with 42 control girls and young women aged 1-25 years who underwent OTC because of cancer. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Follicle density (follicles/mm3), morphology, and health were assessed in ovarian cortex biopsies from TS patients and compared with controls. Hormone concentrations were measured in serum and follicle fluids. Immature cumulus oocyte complexes were obtained and matured in vitro. RESULT(S): Follicles were found in 60% of the biopsies (9 of 15) from TS ovaries. In 78% of the ovaries (7 of 9) with follicles, the follicle density was within the 95% confidence interval of the control group. There was a high rate of abnormal follicle morphology. Six follicle-specific proteins were expressed similarly in TS and control ovaries. However, apoptosis and zona pellucida protein expression were found to be abnormal in TS. Turner syndrome follicle fluid from small antral follicles had lower concentrations of estrogen and testosterone and higher concentrations of antimüllerian hormone than controls. Thirty-one cumulus oocyte complexes were collected from one patient and cultured for 48 hours in vitro, resulting in five metaphase II oocytes (maturation rate 16%, degeneration rate 19%). CONCLUSION(S): The benefits of OTC may be limited to a highly selected group of TS mosaic patients in whom a sizeable pool of normal follicles is present at OTC.
Authors: L Strypstein; E Van Moer; J Nekkebroeck; I Segers; H Tournaye; I Demeestere; M-M Dolmans; W Verpoest; M De Vos Journal: J Assist Reprod Genet Date: 2022-02-05 Impact factor: 3.412
Authors: M J Schleedoorn; B H Mulder; D D M Braat; C C M Beerendonk; R Peek; W L D M Nelen; E Van Leeuwen; A A E M Van der Velden; K Fleischer; On Behalf Of The Turner Fertility Expert Panel Journal: Hum Reprod Date: 2020-05-01 Impact factor: 6.918
Authors: Myra Schleedoorn; Janielle van der Velden; Didi Braat; Ina Beerendonk; Ron van Golde; Ron Peek; Kathrin Fleischer Journal: BMJ Open Date: 2019-12-11 Impact factor: 2.692