Anna van der Veen1, Martijn van Faassen2, Wilhelmina H A de Jong3, André P van Beek4, D A Janneke Dijck-Brouwer5, Ido P Kema6. 1. Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Electronic address: a.van.der.veen03@umcg.nl. 2. Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Electronic address: h.j.r.van.faassen@umcg.nl. 3. Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Electronic address: w.h.a.de.jong@umcg.nl. 4. Department of Endocrinology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Electronic address: a.p.van.beek@umcg.nl. 5. Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Electronic address: d.a.j.dijck@umcg.nl. 6. Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Electronic address: i.p.kema@umcg.nl.
Abstract
BACKGROUND: With liquid chromatography-tandem mass spectrometry (LC-MS/MS) increasingly being used for the quantification of steroid hormones, there is a need for studies that re-establish reference intervals and biological variation in well-defined cohorts. METHODS: A plasma steroid hormone profiling method using LC-MS/MS for quantification of progesterone, 17-hydroxyprogesterone, androstenedione, testosterone and dihydrotestosterone was developed and validated. For reference interval assessment, 280 well-characterized healthy subjects from the LifeLines cohort were selected, including 40 women using oral contraceptive pills (OCP). The biological variation was examined in 30 healthy individuals. Samples were collected over a period of 4 months with 4 week intervals. RESULTS: The developed method proved to be robust and sensitive. The reference interval levels in men are higher, whereas in women the levels tend to decrease with increasing age. In addition, women using OCP had lower levels of 17-OH-progesterone and androstenedione. The biological variation is generally higher in women compared to men, especially with regard to the inter-individual variation. CONCLUSIONS: The gender-specific determination of the reference intervals, together with the observation that the biological variation demonstrated a high degree of variation, allows interpretation of data on individual and group level for improved biochemical characterization of patients in clinical practice.
BACKGROUND: With liquid chromatography-tandem mass spectrometry (LC-MS/MS) increasingly being used for the quantification of steroid hormones, there is a need for studies that re-establish reference intervals and biological variation in well-defined cohorts. METHODS: A plasma steroid hormone profiling method using LC-MS/MS for quantification of progesterone, 17-hydroxyprogesterone, androstenedione, testosterone and dihydrotestosterone was developed and validated. For reference interval assessment, 280 well-characterized healthy subjects from the LifeLines cohort were selected, including 40 women using oral contraceptive pills (OCP). The biological variation was examined in 30 healthy individuals. Samples were collected over a period of 4 months with 4 week intervals. RESULTS: The developed method proved to be robust and sensitive. The reference interval levels in men are higher, whereas in women the levels tend to decrease with increasing age. In addition, women using OCP had lower levels of 17-OH-progesterone and androstenedione. The biological variation is generally higher in women compared to men, especially with regard to the inter-individual variation. CONCLUSIONS: The gender-specific determination of the reference intervals, together with the observation that the biological variation demonstrated a high degree of variation, allows interpretation of data on individual and group level for improved biochemical characterization of patients in clinical practice.
Authors: Anouk E de Wit; Erik J Giltay; Marrit K de Boer; Fokko J Bosker; Aviva Y Cohn; Willem A Nolen; Ursula B Kaiser; Hadine Joffe; Brenda W J H Penninx; Robert A Schoevers Journal: Transl Psychiatry Date: 2021-02-12 Impact factor: 6.222
Authors: Suzanne P Stam; Annet Vulto; Michel J Vos; Michiel N Kerstens; Abraham Rutgers; Ido Kema; Daan J Touw; Stephan Jl Bakker; André P van Beek Journal: BMJ Open Date: 2022-04-26 Impact factor: 3.006