Epidemiological and clinical characteristics of Streptococcus tigurinus endocarditis.

BACKGROUND: Streptococcus tigurinus was recently described as a new streptococcal species within the viridans group streptococci (VGS). The objectives of the present work were to analyse the clinical and microbiological characteristics of S. tigurinus isolated from patients with bacteraemias, to determine the prevalence of S. tigurinus among VGS endocarditis in Spain, and to compare the clinical characteristics and outcomes of endocarditis caused by S. tigurinus and other VGS.
METHODS: Retrospective nationwide study, performed between 2008 and 2016 in 9 Spanish hospitals from 7 different provinces comprising 237 cases of infective endocarditis. Streptococcal isolates were identified by sequencing fragments of their 16S rRNA, sodA and groEL genes. Clinical data of patients with streptococcal endocarditis were prospectively collected according to a pre-established protocol.
RESULTS: Patients with endocarditis represented 7/9 (77.8%) and 26/86 (30.2%) of the bacteraemias caused by S. tigurinus and other VGS, respectively (p < 0.001), in two of the hospital participants. Among patients with streptococcal endocarditis, 12 different Streptococcus species were recognized being S. oralis, S. tigurinus and S. mitis the three more common. No relevant statistical differences were observed in the clinical characteristics and outcomes of endocarditis caused by the different VGS species.
CONCLUSIONS: In this multicenter study performed in Spain, S. tigurinus showed a higher predilection for the endocardial endothelium as compared to other VGS. However, clinical characteristics and outcomes of endocarditis caused by S. tigurinus did not significantly differ from endocarditis caused by other oral streptococci.

Background

Streptococcus tigurinus was firstly described as a new streptococcal species in 2012 based on DNA hybridization analysis and 16S rRNA sequencing, being genetically very similar to Streptococcus oralis [1]. In fact, in 2016, and based on whole-genome sequencing, it was proposed to classify S. tigurinus as a Streptococcus oralis subspecies [2]. S. tigurinus, as other mitis group streptococci has been found as a commensal of the human oral cavity although since its initial description it has been also documented as a causative agent of infective endocarditis (IE) and other infections as meningitis, spondylodiscitis, osteomyelitis, prosthetic infections, etc. [1, 3–7]. The objective of the present study was to establish the clinical and microbiological characteristics of S. tigurinus isolated from patients with bacteraemias, to determine the prevalence of S. tigurinus among the cases of viridans group streptococci (VGS) endocarditis in Spain, and to compare the outcomes of IE caused by S. tigurinus and by other VGS.

Methods

S. tigurinus prevalence study

To study the relevance of finding S. tigurinus in a blood culture, all VGS isolated from blood cultures collected between 2008 and 2016 in two hospitals from Barcelona and San Sebastian, two cities from the north of Spain separated more than 500 km apart, were classified by phenotypic methods and all S. oralis were further identified by gene sequencing.

S. tigurinus endocarditis study

To analyse the characteristics of IE caused by S. tigurinus, a retrospective nationwide study, performed between 2008 and 2016 in 9 Spanish hospitals located in 7 different provinces comprising 237 cases of IE was performed. Diagnosis of IE was done according to the revised Duke diagnostic criteria [8]. Clinical IE data were prospectively collected according to a pre-established protocol [9]. Only cases with definite IE diagnosis were included in the study.

Microbiological techniques

VGS isolates were identified by sequencing fragments of their 16S rRNA, sodA and groEL genes and comparing them with those available at the NCBI and LeBibi databases [10]. A similitude of > 99% with the 3 genes was considered for a correct species identification. All S. tigurinus detected were genotyped by MLST according to the established protocol at the Oral Streptococcus MLST Database web page (https://pubmlst.org/oralstrep). Antimicrobial MICs were determined by the broth microdilution method using Iso-Sensitest Broth (Oxoid) supplemented with lysed horse blood 5% v/v. After incubation for 24 h at 35 °C, susceptibility results were read and interpreted according to CLSI guidelines. S. pneumoniae ATCC 49619 was used as control.

Statistical analysis

The unpaired t-test or the chi-square test (Fisher’s exact test when appropriate) was used to compare continuous and categorical variables, respectively. All statistical analyses were performed using the online available GraphPad software (www.graphpad.com/quickcalcs/).

Ethical considerations

The project and the common case report form were approved by the national and local institutional review boards and ethics committees (E.C. 18/07) and all patients gave their written informed consent to participate in the study.

Results

In the present work, 169 VGS isolates were identified by molecular methods: 95 from the prevalence study and 74 from the study of IE. The 16S rRNA gene correctly identified all of them, sodA misidentified one S. oralis isolate as S. tigurinus and groEL misidentified 5 S. oralis isolates as S. tigurinus (n = 2), S. cristatus (n = 2) and S. mitis (n = 1).

Overall, 95 cases of VGS bacteraemias recorded in the hospitals of Barcelona and San Sebastian were studied, being 9 identified as S. tigurinus. Patients with endocarditis represented 7/9 (77.8%) of the S. tigurinus and 26/86 (30.2%) of the remaining VGS (p = 0.008). This data suggests a bigger attraction of S. tigurinus for the endocardial endothelium as compared with related species of the VGS, a finding that has been also observed in other studies [6].

Of the 237 IE caused by VGS recorded in the study, 74 isolates were available for further studies and were identified by phenotypic methods and by gene sequencing. In global, 12 different Streptococcus species were recognized being S. oralis, S. tigurinus and S. mitis the three more common causing 37.8, 23.0 and 21.6% of IE cases respectively (Table 1). All S. tigurinus had been previously identified by phenotypic methods as S. oralis and were found in 5/9 hospitals and in 4/7 provinces. All S. tigurinus were fully susceptible to oral penicillin, amoxicillin, and cefotaxime except one isolate that had a penicillin MIC = 0.12 mg/L. Three isolates were tetracycline-resistant (MIC> 4 mg/L) and another three erythromycin-resistant (MIC = 2 mg/L). All S. tigurinus isolates were susceptible to clindamycin, levofloxacin and vancomycin. A large heterogeneity of S. tigurinus was observed by MLST, having all different ST. Among the 17 S. tigurinus isolates, there were only two ST previously defined in the MLST database, ST30 and ST65, both previously identified as S. oralis from patients with gingivitis. Comparing IE caused by S. tigurinus and other VGS, patients´ average age was higher for S. tigurinus endocarditis, without statistical significance (p = 0.179 compared to S. mitis) (Table 2). No relevant statistical differences were observed in the clinical characteristics of IE caused by the different VGS species. Left heart valves were more frequently affected in S. tigurinus IE as well as in other streptococci: 45.9% mitral, 31.1% aortic, and 13.6% both valves. Considering all VGS IE, perforation was the most common intracardiac complication (16.2%), followed by abscess (10.8%) and pseudoaneurysm alone (2.7%) or with perforation (2.7%). In-hospital overall mortality in VGS IE (10.8%) was relatively high as compared with other studies [11]. Mortality due to S. tigurinus was quite similar to that of S. oralis (11.3% vs 14.8%, p = 1). Surprisingly, no mortality was recorded for any of the 16 patients with S. mitis IE that was also the group with less patients requiring surgical treatment. Of the patients with IE caused by VGS, 22 were treated only with betalactams (20 with ceftriaxone, 1 with ceftriaxone and ampicillin and 1 with imipenem), 26 with betalactams and gentamicin, being ceftriaxone-gentamicin the combination most frequently used (in 10 patients ceftriaxone-gentamicin alone and in another 8 with a third antibiotic). There were no differences in the antibiotic treatment despite the species identified.

Table 1

Species of viridans group streptococci causing infective endocarditis in Spain, 2008–2016

Species	n	%
S. oralis	28	37.8%
S. tigurinus	17	23.0%
S. mitis	16	21.6%
S. parasanguinis	3	4.1%
S. sanguinis	2	2.7%
S. pneumoniae	2	2.7%
S. infantis	1	1.4%
S. salivarius	1	1.4%
S. infantarius	1	1.4%
S. gordonii	1	1.4%
S. anginosus	1	1.4%
S. alactolyticus	1	1.4%
Total	74

Table 2

Clinical data of patients with infective endocarditis caused by S. mitis, S. oralis, S. tigurinus and other viridans group streptococci, Spain, 2008–2016

	S. mitis (n = 16)	S. oralis (n = 28)	S. tigurinus (n = 17)	Other VGS (n = 13)
Demographics
Age in years: average ± SD (range)	58.4 ± 17.5 (35–94)	60.0 ± 16.7 (32–86)	66.7 ± 17.2 (19–87)	61.2 ± 13.9 (28–80)
Female/Male	2/14	4/24	6/11	5/8
Underlying conditions
Diabetes mellitus	2	2	0	0
Renal insufficiency	0	1	2	1
Pulmonary disease	3	2	3	1
Neoplasm	2	2	3	1
HIV infection	0	3	0	1
Risk factors
Previous infective endocarditis	2	5	2	1
Heart failure	1	4	4	2
Atrial fibrillation	3	4	2	1
Site of acquisition
Nosocomial	0	0	0	0
Community -acquired	15	27	16	12
Health care-related	1	1	1	1
Symptoms at admission
Affected valve
Aortic	6	14	7	7
Mitral	6	9	6	2
Aortic + mitral	2	3	2	2
Tricuspid	1	0	0	0
Tricuspid + mitral	0	0	0	1
Aortic + tricuspid + mitral	0	1	0	0
Pulmonary	1	0	0	0
Ductus arteriosus	0	0	0	1
Not determined	0	1	2	0
Presentation
Fever> 38 °C	16	23	13/16a	11
Splinter hemorrhages	1	0	0	2
Osler nodes	0	0	0	1
Janeway lesions	1	0	0	1
New murmur	3	15	5/16	8
Worsening of old murmur	0	4/24	1/15	0
Protein C reactive: average ± SD (range)	74.2 ± 41.5 (13–168)	55.7 ± 45.6 (1–138)	52.3 ± 96.4 (3–356)	40.8 ± 42.7 (3–101)
Elevated Rheumatoid factor	0/2	4/11	1/4	2/4
Vegetations
Not found	6	6	6	4
Aortic	4	10	6	5
Mitral	3	7	3	1
Tricuspid	1	0	0	0
Aortic + mitral	1	5	2	2
Mitral + tricuspid	0	0	0	1
Chordae tendinae	1	0	0	0
Intracardiac complications
Perforation	2	4	3	3
Abscess	1	4	1	2
Pseudoaneurysm	0	1	0	1
Pseudoaneurysm & perforation	0	2	0	0
NC	0	1	0	2
Clinical course
Embolism	1	6/27a	5/16a	4
New heart failure	1	12/27a	5	4
Persistent bacteraemia	1	0	0	1
Surgery
Indicated	5	19/27a	9	7
Performed	5	18/27a	6	6
Criteria for surgeryb
Cardiac insufficiency	2	9	2	3
Early prosthetic IE	0	1	0	0
Late prosthetic IE	1	2	0	0
Valve insufficiency	3	10	3	4
Embolisms	0	1	0	1
Others	1	5	3	1
Outcome
Days hospital stay: average ± SD (range)	32.4 ± 19.3 (9–85)	31.0 ± 21.8 (3–106)	29.3 ± 17.9 (5–74)	29.9 ± 16.3 (6–53)
In- hospital mortality	0	4	2	2
1-year mortalityc	0/14	3/22	2/15	1/8
Recurrence	0	2	2	0
Antibiotic treatment
Beta-lactams alone	6	9	6	5
Beta-lactams + gentamicin	8	12	7	6
Other combinations	2	7	4	2

aDenominator adjusted to patients with data available

bSome patients had more than one criteria for surgery

cExcluding patents died at hospital

Discussion

Despite the advances in imaging (echocardiography and nuclear medicine), molecular microbiology and surgery, IE is still today a serious disease with high morbidity and mortality rates. In high-income countries, epidemiology of IE is changing with an increase of elderly patients with prosthetic valves or implantable cardiovascular devices [11]. Also, etiologic agents causing IE seems to be changing, with an increase of staphylococcal IE and a reduction of IE caused by VGS [11, 12]. In this Spanish multicenter study, it was previously shown that VGS represented 27.5% cases of definitive IE [13]. In the present work it has been shown that S. tigurinus was responsible for 20% of these definitive IE cases caused by VGS.

S. tigurinus has been associated to IE since its first description, although it has been also described as causing meningitis, spondylodiscitis, prosthetic infections, osteomyelitis, and periodontitis among others. However, in a recent review, IE was the most commonly reported manifestation of S. tigurinus infection [6] probably because IE has been more systematically searched for than other kind of infections. In that review, no deaths were documented among patients with S. tigurinus infection except for one case of osteomyelitis. In our study, 2 patients with S. tigurinus IE died during admission with no difference in mortality rates to IE caused by other VGS.

Besides to the oral origin of S. tigurinus infections, an enteric source has been also postulated after the translocation of the pathogen from an intraabdominal disorder [14]. However we considered that in most of our patients the origin was the oral cavity as no intraabdominal condition was found in any of the patients, whether the causative agent of the IE was S. tigurinus or another of the VSG commonly found in the oral mucosa. The oral origin of most VGS IE highlights the need of an exquisite dental care in patients with risk for IE [15].

IE caused by S. tigurinus did not clinically differ from IE caused by other VGS, showing a community-acquired origin, clinical course and outcomes in general better than bacterial IE caused by other Gram-positive bacteria as Staphylococcus aureus or Enterococcus [11]. Despite the wide genomic heterogeneity most isolates were fully susceptible to commonly used antibiotics in the treatment of IE. An endocarditis should always be suspected when a S. tigurinus is isolated from a blood culture due to the high prevalence of IE caused by this, otherwise commensal bacteria.

Conclusion

In this multicenter study performed in Spain, S. tigurinus was a common cause of IE. Clinical characteristics and outcomes of S. tigurinus endocarditis did not differ from endocarditis caused by other VGS. S. tigurinus showed a high genomic heterogeneity with most isolates susceptible in vitro to antibiotics commonly used in the treatment of IE.