Marek Lipiec1, Łukasz Radulski2, Krzysztof Szulowski3. 1. National Veterinary Research Institute, Pulawy, Poland. mlipiec@piwet.pulawy.pl. 2. National Veterinary Research Institute, Pulawy, Poland. lukasz.radulski@piwet.pulawy.pl. 3. National Veterinary Research Institute, Pulawy, Poland. kszjanow@piwet.pulawy.pl.
Abstract
INTRODUCTION: Bovine tuberculosis is a chronic contagious disease caused by Mycobacterium bovis or Mycobacterium caprae. Before widespread action conducted in Poland between 1959-1975 to combat bovine tuberculosis (BTB), about 40% of all tuberculosis cases in pigs was caused by the bovine bacillus. At the present time, correctly carried out, long-term control of cattle has resulted in cases of bovine tuberculosis in pigs and humans being extremely rare and sporadic. In pigs, tuberculosis is most often caught in a slaughterhouse during slaughter. MATERIAL AND METHODS: Samples came from pigs kept on the farm. Traditional bacteriological methods on solid media (Stonebrink, LJ with pyruvate) supported by the semi-automatic, liquid indicative culture method (MGIT) and PCR test were applied in targeted studies. The GenoType Mycobacterium MTBC and CM tests (Hain Lifescience, Germany) were used to additionally confirm that isolated strains classification was used. RESULTS: Strains of mycobacteria were isolated from all examined pigs. Mycobacterium bovis was determined by real time PCR and Hain Genotype methods. CONCLUSIONS: In order to effectively fight against BTB, all animals on farms should be tested, regardless of species, while the milk of suspected cows should be utilized without being used for feed. It is important to adapt the current legal regulations to the current epidemiological situation.
INTRODUCTION:Bovinetuberculosis is a chronic contagious disease caused by Mycobacterium bovis or Mycobacterium caprae. Before widespread action conducted in Poland between 1959-1975 to combat bovinetuberculosis (BTB), about 40% of all tuberculosis cases in pigs was caused by the bovinebacillus. At the present time, correctly carried out, long-term control of cattle has resulted in cases of bovinetuberculosis in pigs and humans being extremely rare and sporadic. In pigs, tuberculosis is most often caught in a slaughterhouse during slaughter. MATERIAL AND METHODS: Samples came from pigs kept on the farm. Traditional bacteriological methods on solid media (Stonebrink, LJ with pyruvate) supported by the semi-automatic, liquid indicative culture method (MGIT) and PCR test were applied in targeted studies. The GenoType Mycobacterium MTBC and CM tests (Hain Lifescience, Germany) were used to additionally confirm that isolated strains classification was used. RESULTS: Strains of mycobacteria were isolated from all examined pigs. Mycobacterium bovis was determined by real time PCR and Hain Genotype methods. CONCLUSIONS: In order to effectively fight against BTB, all animals on farms should be tested, regardless of species, while the milk of suspected cows should be utilized without being used for feed. It is important to adapt the current legal regulations to the current epidemiological situation.
Authors: Monika Krajewska-Wędzina; Michele A Miller; Anna Didkowska; Anna Kycko; Łukasz Radulski; Marek Lipiec; Marcin Weiner Journal: J Vet Res Date: 2022-03-25 Impact factor: 2.058
Authors: Anna Didkowska; Blanka Orłowska; Monika Krajewska-Wędzina; Michał Krzysiak; Małgorzata Bruczyńska; Jan Wiśniewski; Daniel Klich; Wanda Olech; Krzysztof Anusz Journal: Pathogens Date: 2022-02-17