Literature DB >> 3091697

Enzyme-linked immunosorbent assay of mouse interleukin-2 receptors.

H Osawa, O Josimovic-Alasevic, T Diamantstein.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) which measures soluble mouse interleukin-2 receptor (IL-2R) was developed, using two monoclonal anti-mouse IL-2R antibodies directed against two different epitopes of the IL-2R molecule. The ELISA-reactive material was identical with the IL-2R material which was capable of binding to affinity support beads coupled with recombinant interleukin-2 (IL-2). Quantitation of IL-2R in detergent-lysed cells was compared to that of the purified IL-2R, and the detection limit was estimated to be within the range of 2-10 ng/ml of a test sample. This sensitivity made it possible to determine soluble IL-2R levels in cell culture supernatants and mouse sera.

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Year:  1986        PMID： 3091697     DOI： 10.1016/0022-1759(86)90510-7

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  7 in total

1.  Demonstration of two distinct forms of released low affinity-type rat IL-2 receptors.

Authors:  T Herrmann; O Josimovic-Alasevic; A Mouzaki; T Diamantstein
Journal:  Immunology       Date:  1989-03       Impact factor: 7.397

2.  The therapeutic efficacy of an anti-IL-2 receptor monoclonal antibody correlates with an increase in serum soluble IL-2 receptor levels.

Authors:  H D Volk; O Josimovic-Alasevic; M Gross; T Diamantstein
Journal:  Clin Exp Immunol       Date:  1989-04       Impact factor: 4.330

3.  Monitoring of interleukin-2 receptor (IL-2R) expression in vivo and studies on an IL-2R-directed immunosuppressive therapy of active and adoptive adjuvant-induced arthritis in rats.

Authors:  K G Stünkel; P Theisen; A Mouzaki; T Diamantstein; H D Schlumberger
Journal:  Immunology       Date:  1988-08       Impact factor: 7.397

4.  Interleukin 2 receptor in patients with localized and systemic parasitic diseases.

Authors:  O Josimovic-Alasevic; H Feldmeier; K Zwingenberger; G Harms; H Hahn; M Shrisuphanunt; T Diamantstein
Journal:  Clin Exp Immunol       Date:  1988-05       Impact factor: 4.330

5.  Interleukin-2 receptors in experimental Chagas' disease.

Authors:  D R Pakianathan; R E Kuhn
Journal:  Infect Immun       Date:  1992-09       Impact factor: 3.441

6.  Soluble interleukin 2 receptors are released from the cell surface of normal murine B lymphocytes stimulated with interleukin 5.

Authors:  M S Loughnan; C J Sanderson; G J Nossal
Journal:  Proc Natl Acad Sci U S A       Date:  1988-05       Impact factor: 11.205

7.  Determination of cell-free interleukin 2 receptor level in the serum of normal animals and of animals bearing IL-2 receptor positive tumours with high or low metastatic capacity.

Authors:  V Schirrmacher; O Josimovic-Alasevic; H Osawa; T Diamantstein
Journal:  Br J Cancer       Date:  1987-06       Impact factor: 7.640
  7 in total

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