Niels Belmans1,2, Liese Gilles1, Piroska Virag3, Mihaela Hedesiu3, Benjamin Salmon4, Sarah Baatout2, Stéphane Lucas5, Reinhilde Jacobs6,7, Ivo Lambrichts1, Marjan Moreels2. 1. Morphology Group, Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium. 2. Belgian Nuclear Research Centre, Radiobiology Unit, SCK•CEN, Mol, Belgium. 3. 'Iuliu Hatieganu' University of Medicine and Pharmacy, Department of Oral and Maxillofacial Radiology, Cluj-Napoca, Romania. 4. Department of Orofacial Pathologies, Imaging and Biotherapies Lab and Dental Medicine, Paris Descartes University - Sorbonne Paris Cité, Bretonneau Hospital, HUPNVS, AP-HP, Paris, France. 5. University of Namur, Research Institute for Life Sciences, Namur, Belgium. 6. Department of Imaging and Pathology Katholieke Universiteit Leuven, OMFS IMPATH Research Group, and University Hospitals, Oral and Maxillofacial Surgery, Dentomaxillofacial Imaging Center, Leuven, Belgium. 7. Department Dental Medicine, Karolinska Institutet, Huddinge, Sweden.
Abstract
OBJECTIVES: Cone-beam CT (CBCT) is a medical imaging technique used in dental medicine. However, there are no conclusive data available indicating that exposure to X-ray doses used by CBCT are harmless. We aim, for the first time, to characterize the potential age-dependent cellular and subcellular effects related to exposure to CBCT imaging. Current objective is to describe and validate the protocol for characterization of cellular and subcellular changes after diagnostic CBCT. METHODS: Development and validation of a dedicated two-part protocol: 1) assessing DNA double strand breaks (DSBs) in buccal mucosal (BM) cells and 2) oxidative stress measurements in saliva samples. BM cells and saliva samples are collected prior to and 0.5 h after CBCT examination. BM cells are also collected 24 h after CBCT examination. DNA DSBs are monitored in BM cells via immunocytochemical staining for γH2AX and 53BP1. 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) and total antioxidant capacity are measured in saliva to assess oxidative damage. RESULTS: Validation experiments show that sufficient BM cells are collected (97.1 ± 1.4 %) and that γH2AX/53BP1 foci can be detected before and after CBCT examination. Collection and analysis of saliva samples, either sham exposed or exposed to IR, show that changes in 8-oxo-dG and total antioxidant capacity can be detected in saliva samples after CBCT examination. CONCLUSION: The DIMITRA Research Group presents a two-part protocol to analyze potential age-related biological differences following CBCT examinations. This protocol was validated for collecting BM cells and saliva and for analyzing these samples for DNA DSBs and oxidative stress markers, respectively.
OBJECTIVES: Cone-beam CT (CBCT) is a medical imaging technique used in dental medicine. However, there are no conclusive data available indicating that exposure to X-ray doses used by CBCT are harmless. We aim, for the first time, to characterize the potential age-dependent cellular and subcellular effects related to exposure to CBCT imaging. Current objective is to describe and validate the protocol for characterization of cellular and subcellular changes after diagnostic CBCT. METHODS: Development and validation of a dedicated two-part protocol: 1) assessing DNA double strand breaks (DSBs) in buccal mucosal (BM) cells and 2) oxidative stress measurements in saliva samples. BM cells and saliva samples are collected prior to and 0.5 h after CBCT examination. BM cells are also collected 24 h after CBCT examination. DNA DSBs are monitored in BM cells via immunocytochemical staining for γH2AX and 53BP1. 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) and total antioxidant capacity are measured in saliva to assess oxidative damage. RESULTS: Validation experiments show that sufficient BM cells are collected (97.1 ± 1.4 %) and that γH2AX/53BP1 foci can be detected before and after CBCT examination. Collection and analysis of saliva samples, either sham exposed or exposed to IR, show that changes in 8-oxo-dG and total antioxidant capacity can be detected in saliva samples after CBCT examination. CONCLUSION: The DIMITRA Research Group presents a two-part protocol to analyze potential age-related biological differences following CBCT examinations. This protocol was validated for collecting BM cells and saliva and for analyzing these samples for DNA DSBs and oxidative stress markers, respectively.
Authors: Niels Belmans; Anne Caroline Oenning; Benjamin Salmon; Bjorn Baselet; Kevin Tabury; Stéphane Lucas; Ivo Lambrichts; Marjan Moreels; Reinhilde Jacobs; Sarah Baatout Journal: Dentomaxillofac Radiol Date: 2021-05-14 Impact factor: 3.525