Maria A Jure1, Marta E Castillo2, Humberto E Musa3, Carolina G López3, Mariel A Cáceres4, Silvana D Mochi4, Aurore A Bousquet5, Nathalie A Genel6, Guillaume A Arlet7, Dominique C Decré7. 1. Cátedra de Bacteriologia, Instituto de Microbiología Luis C, Verna. Fac. de Bioqca, Qca y Fcia. Universidad Nacional de Tucumán, Ayacucho 491, San Miguel de Tucumán, CP 4000, Argentina; Centro de Microbiología Médica. Rondeau 877, San Miguel de Tucumán, CP 4000 Argentina. Electronic address: majure.fbqf.unt.edu@gmail.com. 2. Cátedra de Bacteriologia, Instituto de Microbiología Luis C, Verna. Fac. de Bioqca, Qca y Fcia. Universidad Nacional de Tucumán, Ayacucho 491, San Miguel de Tucumán, CP 4000, Argentina. 3. Centro de Microbiología Médica. Rondeau 877, San Miguel de Tucumán, CP 4000 Argentina. 4. Hospital Angel Cruz Padilla, Alberdi 540, 4000 San Miguel de Tucumán, CP 4000 Argentina. 5. Sorbonne Universités, UPMC Univ Paris 06 CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, team E13 (Bacteriology), Paris, F-75013, France. 6. Sorbonne Universités, UPMC Univ Paris 06 CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, team E13 (Bacteriology), Paris, F-75013, France; INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI, team E13 (Bacteriology), Paris, F-75013, France. 7. Sorbonne Universités, UPMC Univ Paris 06 CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, team E13 (Bacteriology), Paris, F-75013, France; INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI, team E13 (Bacteriology), Paris, F-75013, France; Assistance Publique des Hôpitaux de Paris, Hôpitaux Universitaires Est Parisiens, Département de Bactériologie, Paris, France.
Abstract
BACKGROUND: In Argentina, there has been an abrupt increase in KPC-2-producing Klebsiella pneumoniae (K. pneumoniae). Tucumán is a multi-border area, so the rapid dissemination of carbapenem-resistant K. pneumoniae is a clinically relevant problem for the region. OBJECTIVES: This study aimed to investigate the epidemiological and molecular patterns of KPC-producing K. pneumoniae clinical isolates collected from different hospitals in Tucumán. METHODS: Carbapenem-resistant K. pneumoniae strains were sequentially and uniquely collected during two time periods. Antibiotic susceptibility was determined by the automated Vitex 2® system and using the standard agar dilution test. Multilocus sequence typing and pulsed-field electrophoresis were used for epidemiological analysis. The genetic structures around blaKPC and the encoding genes of extended-spectrum β-lactamases were detected by polymerase chain reaction and sequencing. Plasmids were analysed by conjugation and using the plasmid relaxase gene-typing method. RESULTS: All 37 isolates were multidrug resistant, and theblaKPC-2 gene was confirmed in all of them. In 17 isolates (45.9%), the blaCTX-M-2 gene was also amplified, as well as blaSHV-2 in five isolates (13.5%) and blaCTX-M-2/blaSHV-2 in four isolates (10.8%). The molecular epidemiology of the blaKPC-2 gene has resulted in it being associated with an IncL/M transferable plasmid disseminating in various sequence types (STs) (ST17, ST556, ST342, ST147, ST461, ST65, ST15 and ST70), and in a new genetic environment with a 764-bp deletion in the ISKpn7-blaKPC region. CONCLUSIONS: These findings contribute to the understanding of the great diversity of the blaKPC-2-carrying genetic platforms.
BACKGROUND: In Argentina, there has been an abrupt increase in KPC-2-producing Klebsiella pneumoniae (K. pneumoniae). Tucumán is a multi-border area, so the rapid dissemination of carbapenem-resistant K. pneumoniae is a clinically relevant problem for the region. OBJECTIVES: This study aimed to investigate the epidemiological and molecular patterns of KPC-producing K. pneumoniae clinical isolates collected from different hospitals in Tucumán. METHODS:Carbapenem-resistant K. pneumoniae strains were sequentially and uniquely collected during two time periods. Antibiotic susceptibility was determined by the automated Vitex 2® system and using the standard agar dilution test. Multilocus sequence typing and pulsed-field electrophoresis were used for epidemiological analysis. The genetic structures around blaKPC and the encoding genes of extended-spectrum β-lactamases were detected by polymerase chain reaction and sequencing. Plasmids were analysed by conjugation and using the plasmid relaxase gene-typing method. RESULTS: All 37 isolates were multidrug resistant, and theblaKPC-2 gene was confirmed in all of them. In 17 isolates (45.9%), the blaCTX-M-2 gene was also amplified, as well as blaSHV-2 in five isolates (13.5%) and blaCTX-M-2/blaSHV-2 in four isolates (10.8%). The molecular epidemiology of the blaKPC-2 gene has resulted in it being associated with an IncL/M transferable plasmid disseminating in various sequence types (STs) (ST17, ST556, ST342, ST147, ST461, ST65, ST15 and ST70), and in a new genetic environment with a 764-bp deletion in the ISKpn7-blaKPC region. CONCLUSIONS: These findings contribute to the understanding of the great diversity of the blaKPC-2-carrying genetic platforms.
Authors: Leonardo Albarracin; Ramiro Ortiz Moyano; Juan Martin Vargas; Bruno G N Andrade; Juan Cortez Zamar; Stefania Dentice Maidana; Kohtaro Fukuyama; Shoichiro Kurata; María Ángela Jure; Haruki Kitazawa; Julio Villena Journal: Int J Mol Sci Date: 2022-07-01 Impact factor: 6.208
Authors: K A Strydom; L Chen; M M Kock; A C Stoltz; G Peirano; D B Nobrega; M Lowe; M M Ehlers; N M Mbelle; B N Kreiswirth; J D D Pitout Journal: J Antimicrob Chemother Date: 2020-04-01 Impact factor: 5.790