Weihua Miao1, Huibing Gao2, Xiaojin Hou3. 1. a Department of Orthopedic Surgery , Heze City Hospital , Heze city , Shandong province , China. 2. b Department of Head and Neck Oncology , Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences , Guangzhou , Guangdong Province , China. 3. c Department of Orthopedics , Xianyang Hospital of Yan'an University , Xianyang city , Shanxi province , China.
Abstract
Purpose: Titanium particle-induced osteolysis is one of the important causes of aseptic loosening of artificial joints. Previous studies have shown the potential of natural compounds in preventing Ti particle-induced bone resorption. In this study, we observed the effects of magnesium lithospermate B (MLB) on titanium particle-induced osteoclast activity in vitro. Materials and Methods: RAW264.7 cells were treated with titanium particles (0.1 mg/mL) in the presence or absence of MLB (200 nmol/L). We evaluated the osteoclast formation, bone pits formation and tartrate-resistant acid phosphatase 5b (Tracp5b) levels. Reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to evaluate osteoclast differentiation-related genes (TRAF6, NFATc1, and c-fos) and protein expression. Results: The number of osteoclasts, pit formation and Tracp5b levels were all the group treated with titanium particles compared to the control group (all p < 0.05). Titanium particles also promoted the expression of the TRAF6, NFATc1 and c-fos genes and protein expression. MLB significantly abolished the titanium particle-enhanced osteoclast and pits formation, and Traf6, NFATc1, and c-fos expression. Conclusions: Our data demonstrated that MLB can suppress titanium-induced osteoclast activity via inhibiting c-fos and NFATc1 expression.
Purpose: Titanium particle-induced osteolysis is one of the important causes of aseptic loosening of artificial joints. Previous studies have shown the potential of natural compounds in preventing Ti particle-induced bone resorption. In this study, we observed the effects of magnesium lithospermate B (MLB) on titanium particle-induced osteoclast activity in vitro. Materials and Methods: RAW264.7 cells were treated with titanium particles (0.1 mg/mL) in the presence or absence of MLB (200 nmol/L). We evaluated the osteoclast formation, bone pits formation and tartrate-resistant acid phosphatase 5b (Tracp5b) levels. Reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to evaluate osteoclast differentiation-related genes (TRAF6, NFATc1, and c-fos) and protein expression. Results: The number of osteoclasts, pit formation and Tracp5b levels were all the group treated with titanium particles compared to the control group (all p < 0.05). Titanium particles also promoted the expression of the TRAF6, NFATc1 and c-fos genes and protein expression. MLB significantly abolished the titanium particle-enhanced osteoclast and pits formation, and Traf6, NFATc1, and c-fos expression. Conclusions: Our data demonstrated that MLB can suppress titanium-induced osteoclast activity via inhibiting c-fos and NFATc1 expression.