| Literature DB >> 3090401 |
T J Harris, T Patel, F A Marston, S Little, J S Emtage, G Opdenakker, G Volckaert, W Rombauts, A Billiau, P De Somer.
Abstract
cDNA clones of the mRNA coding for tissue-type plasminogen activator (t-PA) have been obtained and their nucleotide sequences compared to those reported previously. A gene coding for t-PA has been reconstructed and inserted into vectors for expression in prokaryotic cells. Relatively high levels of t-PA accumulated in inclusion bodies in Escherichia coli containing an optimized expression plasmid, but only a small proportion of the insoluble protein was recovered as active enzyme using a variety of solubilization procedures.Entities:
Mesh：
Substances：
Year: 1986 PMID： 3090401
Source DB: PubMed Journal: Mol Biol Med ISSN： 0735-1313