Mohsen Chamanara1, Alireza Abdollahi2, Seyed Mahdi Rezayat3, Mamoud Ghazi-Khansari3, Ahmadreza Dehpour3,4, Ehsan Nassireslami1, Amir Rashidian5,6. 1. Department of Pharmacology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran. 2. Department of Pathology, Imam Hospital, Tehran University of Medical Sciences, Tehran, Iran. 3. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 4. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran. 5. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. A_rashidian@sina.tums.ac.ir. 6. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran. A_rashidian@sina.tums.ac.ir.
Abstract
AIM: The aim of the present study was to evaluate the anti-inflammatory effect of thymol in acetic acid-induced rat colitis through inhibiting the NF-κB signaling pathway. METHODS: Colitis was induced by intra-rectal administration of 2 mL of diluted acetic acid (4%) solution using a flexible plastic rubber catheter in Wistar rats. Colitis was induced on the first day and all treatments were applied 5 days after the induction of colitis. Thymol was dissolved in 0.2% tween 80 in saline and administered orally at doses of 10, 30, and 100 mg/kg per day. Macroscopic and histopathologic investigations were done. The expression of myeloperoxidase (MPO) and tumor necrosis factor-α (TNF-α) was determined by immunohistochemistry (IHC) assay. The protein expression level of pNF-κB p65 was measured by the Western blot technique. RESULTS: Treatment with thymol reduced mucosal and histological damages compared to the acetic acid group. Our results showed that thymol markedly inhibited the production of MPO and TNF-α in the colon tissue of the acetic acid-induced group. In addition, thymol decreased acetic acid-induced up-regulation of pNFκB p65 protein. CONCLUSIONS: The results of our study suggest that thymol exerts an anti-inflammatory effect in acetic acid-induced rat colitis by inhibiting the NF-κB signaling pathway and downregulating TNF-α and MPO expressions.
AIM: The aim of the present study was to evaluate the anti-inflammatory effect of thymol in acetic acid-induced ratcolitis through inhibiting the NF-κB signaling pathway. METHODS:Colitis was induced by intra-rectal administration of 2 mL of diluted acetic acid (4%) solution using a flexible plastic rubber catheter in Wistar rats. Colitis was induced on the first day and all treatments were applied 5 days after the induction of colitis. Thymol was dissolved in 0.2% tween 80 in saline and administered orally at doses of 10, 30, and 100 mg/kg per day. Macroscopic and histopathologic investigations were done. The expression of myeloperoxidase (MPO) and tumor necrosis factor-α (TNF-α) was determined by immunohistochemistry (IHC) assay. The protein expression level of pNF-κB p65 was measured by the Western blot technique. RESULTS: Treatment with thymol reduced mucosal and histological damages compared to the acetic acid group. Our results showed that thymol markedly inhibited the production of MPO and TNF-α in the colon tissue of the acetic acid-induced group. In addition, thymol decreased acetic acid-induced up-regulation of pNFκB p65 protein. CONCLUSIONS: The results of our study suggest that thymol exerts an anti-inflammatory effect in acetic acid-induced ratcolitis by inhibiting the NF-κB signaling pathway and downregulating TNF-α and MPO expressions.
Authors: Xiaowan Du; Wei Chen; Yufeng Wang; Chen Chen; Lei Guo; Rui Ju; Juan Li; Dechang Zhang; Lei Zhu; Caiying Ye Journal: Int Immunopharmacol Date: 2017-01-31 Impact factor: 4.932
Authors: I Arijs; K Li; G Toedter; R Quintens; L Van Lommel; K Van Steen; P Leemans; G De Hertogh; K Lemaire; M Ferrante; F Schnitzler; L Thorrez; K Ma; X-Y R Song; C Marano; G Van Assche; S Vermeire; K Geboes; F Schuit; F Baribaud; P Rutgeerts Journal: Gut Date: 2009-08-20 Impact factor: 23.059
Authors: Jan Macku; Katerina Kubova; Martina Urbanova; Jan Muselik; Ales Franc; Gabriela Koutna; Miroslava Pavelkova; David Vetchy; Josef Masek; Eliska Maskova; Jiri Brus Journal: Pharmaceutics Date: 2022-07-25 Impact factor: 6.525