| Literature DB >> 30900875 |
Ansha Luthra1,2, David B Langley1, Peter Schofield1,2, Jennifer Jackson1, Mahmoud Abdelatti1, Romain Rouet1, Damien Nevoltris1, Ohan Mazigi1,2, Ben Crossett3, Mary Christie4, Daniel Christ1,2.
Abstract
We developed a repertoire approach to generate human antibody bispecifics. Using phage display selection of antibody heavy chains in the presence of a competitor light chain and providing a cognate light chain with an affinity handle, we identified mutations that prevent heavy/light chain mispairing. The strategy allows for the selection of human antibody chains that autonomously assemble into bispecifics.Entities:
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Year: 2019 PMID： 30900875 DOI： 10.1021/acs.biochem.9b00037
Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162