Yongquan Zheng1, Xiaoqian Zhang2, Jiuxia Chen3, Qi Zhou3, Hongchang Gao3. 1. Department of Pharmacy, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China. 2. Department of Pharmacy, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. 3. School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
Abstract
OBJECTIVE: To investigate the metabolic profiles of urine from APP/PS1 mice with early-stage Alzheimer's disease (AD). METHODS: Urine samples were collected from 13 APP/PS1 mice of 16 weeks and 15 wild-type mice. 1H-NMR spectroscopy was acquired with a one-dimensional NOESY pulse sequence, and the integral values were imported to SIMCA-P+12.0 software for analysis. RESULTS: The metabonomic analysis showed that the metabolic profiles of the APP/PS1 mice were significantly different from that of age-matched wild-type mice. The levels of 3-hydroxybutyrate, 2-hydroxybutyrate, succinic acid, 2-ketoglutaric acid, citric acid, cis-aconitic acid, fumaric acid decreased, and those of acetic acid, trimethylamine, taurine, creatinine, hippuric acid, formic acid, trigonelline, urea increased (all P<0.05). CONCLUSIONS: Metabolic pathways including glucose metabolism and methylamine metabolism may be involved in the pathogenesis of early AD.
OBJECTIVE: To investigate the metabolic profiles of urine from APP/PS1 mice with early-stage Alzheimer's disease (AD). METHODS: Urine samples were collected from 13 APP/PS1 mice of 16 weeks and 15 wild-type mice. 1H-NMR spectroscopy was acquired with a one-dimensional NOESY pulse sequence, and the integral values were imported to SIMCA-P+12.0 software for analysis. RESULTS: The metabonomic analysis showed that the metabolic profiles of the APP/PS1 mice were significantly different from that of age-matched wild-type mice. The levels of 3-hydroxybutyrate, 2-hydroxybutyrate, succinic acid, 2-ketoglutaric acid, citric acid, cis-aconitic acid, fumaric acid decreased, and those of acetic acid, trimethylamine, taurine, creatinine, hippuric acid, formic acid, trigonelline, urea increased (all P<0.05). CONCLUSIONS: Metabolic pathways including glucose metabolism and methylamine metabolism may be involved in the pathogenesis of early AD.