Literature DB >> 30899769

Hypoxia Reporter Element Assay.

Daelynn R Buelow1, Sharyn D Baker1.   

Abstract

Hypoxia is a condition in which there is a decrease in oxygen supply to the cellular environment. Changes to cellular oxygen levels can lead to transcriptional changes of oxygen-regulated genes. Reporter assays are used to study gene expression alteration and modifications in response to environmental changes. Dual-reporter assays allow the simultaneous measurement of two different genes within a single cell, thus improving experimental accuracy. Within this protocol, we describe the utilization of the LightSwitch Dual Assay System to measure BMX expression in response to hypoxic conditions.

Entities:  

Keywords:  Hypoxia; Luciferase; Regulation; Reporter assay; Transcription

Year:  2018        PMID： 30899769      PMCID： PMC6424511          DOI： 10.21769/BioProtoc.2951

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  4 in total

1.  Comprehensive analysis of kinase inhibitor selectivity.

Authors:  Mindy I Davis; Jeremy P Hunt; Sanna Herrgard; Pietro Ciceri; Lisa M Wodicka; Gabriel Pallares; Michael Hocker; Daniel K Treiber; Patrick P Zarrinkar
Journal:  Nat Biotechnol       Date:  2011-10-30       Impact factor: 54.908

2.  Critical function of Bmx/Etk in ischemia-mediated arteriogenesis and angiogenesis.

Authors:  Yun He; Yan Luo; Shibo Tang; Iiro Rajantie; Petri Salven; Matthias Heil; Rong Zhang; Dianhong Luo; Xianghong Li; Hongbo Chi; Jun Yu; Peter Carmeliet; Wolfgang Schaper; Albert J Sinusas; William C Sessa; Kari Alitalo; Wang Min
Journal:  J Clin Invest       Date:  2006-08-24       Impact factor: 14.808

3.  Hypoxia-induced upregulation of BMX kinase mediates therapeutic resistance in acute myeloid leukemia.

Authors:  Jolieke G van Oosterwijk; Daelynn R Buelow; Christina D Drenberg; Aksana Vasilyeva; Lie Li; Lei Shi; Yong-Dong Wang; David Finkelstein; Sheila A Shurtleff; Laura J Janke; Stanley Pounds; Jeffrey E Rubnitz; Hiroto Inaba; Navjotsingh Pabla; Sharyn D Baker
Journal:  J Clin Invest       Date:  2017-12-11       Impact factor: 14.808

4.  Tyrosine kinase BMX phosphorylates phosphotyrosine-primed motif mediating the activation of multiple receptor tyrosine kinases.

Authors:  Sen Chen; Xinnong Jiang; Christina A Gewinner; John M Asara; Nicholas I Simon; Changmeng Cai; Lewis C Cantley; Steven P Balk
Journal:  Sci Signal       Date:  2013-05-28       Impact factor: 8.192
  4 in total
  1 in total

1.  DNA Methylation-Based Epigenetic Repression of SLC22A4 Promotes Resistance to Cytarabine in Acute Myeloid Leukemia.

Authors:  Daelynn R Buelow; Jason T Anderson; Stanley B Pounds; Lei Shi; Jatinder K Lamba; Shuiying Hu; Alice A Gibson; Emily A Goodwin; Alex Sparreboom; Sharyn D Baker
Journal:  Clin Transl Sci       Date:  2020-09-09       Impact factor: 4.689
  1 in total

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