Chenglong Li1, Lixin Ma2, Yongliang Liu1, Zhenzhu Li1, Qingbo Wang1, Zheng Chen1, Xin Geng3, Xinyu Han1, Jingdong Sun4, Zefu Li5. 1. Department of Neurosurgery, Binzhou Medical University Hospital, Binzhou 256603, China. 2. Department of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing 100000, China. 3. Department of Pediatric Surgery, Binzhou Medical University Hospital, Binzhou 256603, China. 4. Department of Pediatric Surgery, Maternity And Child Health Care of Zaozhuang, Zaozhuang 277100, China. 5. Department of Neurosurgery, Binzhou Medical University Hospital, Binzhou 256603, China. Electronic address: lizefu163@163.com.
Abstract
OBJECTIVE: In this study, we aim to evaluate Toll-like receptor 2 (TLR2) expression in human glioma tumors and the correlation between its expression with degrees of malignancy and autophagy, development of tumors. METHOD: Immunohistochemistry and Western blot were carried out to determine the expression of LC3, Beclin1 and TLR2 in 74 glioma specimens. We analyzed the prognosis of 551 glioma patients through the Cancer Genome Atlas (TCGA). To determine the effect of TLR2 in glioma, we manipulated TLR2 expression using TLR2 plasmid transfer technique in U87 human glioma cell. RESULTS: TLR2 expression in high-grade was significantly higher than that in low-grade glioma group (P < 0.05). TLR2 was positively correlated with tumor grade (P < 0.05). Spearman correlation showed that the expression of TLR2 was positively correlated with the numbers of LC3 and Beclin1 (P < 0.05). The patients with high TLR2 expression had a poorer outcome compared with the patients with low TLR2 in low-grade glioma (P < 0.05). TLR2 overexpression enhances glioma cell activity and accelerates cell cycle progression. In addition, treatment with TLR2 overexpression increases the conversion rate of LC3-I to LC3-II and enhances the level of phosphorylated p38. CONCLUSION: TLR2 promotes development and progression of human glioma via enhancing autophagy.
OBJECTIVE: In this study, we aim to evaluate Toll-like receptor 2 (TLR2) expression in humanglioma tumors and the correlation between its expression with degrees of malignancy and autophagy, development of tumors. METHOD: Immunohistochemistry and Western blot were carried out to determine the expression of LC3, Beclin1 and TLR2 in 74 glioma specimens. We analyzed the prognosis of 551 gliomapatients through the Cancer Genome Atlas (TCGA). To determine the effect of TLR2 in glioma, we manipulated TLR2 expression using TLR2 plasmid transfer technique in U87 humanglioma cell. RESULTS:TLR2 expression in high-grade was significantly higher than that in low-grade glioma group (P < 0.05). TLR2 was positively correlated with tumor grade (P < 0.05). Spearman correlation showed that the expression of TLR2 was positively correlated with the numbers of LC3 and Beclin1 (P < 0.05). The patients with high TLR2 expression had a poorer outcome compared with the patients with low TLR2 in low-grade glioma (P < 0.05). TLR2 overexpression enhances glioma cell activity and accelerates cell cycle progression. In addition, treatment with TLR2 overexpression increases the conversion rate of LC3-I to LC3-II and enhances the level of phosphorylated p38. CONCLUSION:TLR2 promotes development and progression of humanglioma via enhancing autophagy.
Authors: Michael Itak Ita; Jiang Huai Wang; André Toulouse; Chris Lim; Noel Fanning; Michael O'Sullivan; Yvonne Nolan; George Finbarr Kaar; Henry Paul Redmond Journal: Acta Neurochir (Wien) Date: 2021-10-13 Impact factor: 2.816