Yolk immunoreactive corticosterone in hierarchical follicles of Japanese quail (Coturnix japonica) exposed to heat challenge.

High temperature decreases the egg number, ovarian weight, and hierarchical follicle number. In the present study, we investigated the effect of high temperature on the quality of eggs of adult female quails. Laying quail were raised under a standard thermal condition of 25 °C until exposed to an elevated temperature of 34 °C (experimental) or maintained at 25 °C (control) from 12:00 to 16:00 for 10 consecutive days. Weight and number of eggs were measured; serum and the largest follicles were collected and used for hormone measurement. Ovaries and adrenals were collected for expression analysis of 3β- and 17β-HSD, genes encoding steroidogenic enzymes. Egg weight slightly decreased with an increase in the treatment time in the heat-challenged group; the egg weight significantly decreased in the heat treatment group than in the control group during the last 2 days of experiment (P < 0.05). The laying rate showed no difference during the experimental period but significantly decreased on the last day in the heat treatment group. In the experimental group the ovaries and oviducts were lighter (P < 0.05) and the hierarchical follicle number and ovarian weight decreased (P < 0.05) compared to the control group. Although serum corticosterone level significantly increased after heat challenge (P < 0.05) and immediately recovered to the normal level, yolk immunoreactive corticosterone in the hierarchical follicle (F1, F2, F3) significantly increased (P < 0.05). The expression level of 17β-HSD showed no changes in the ovary but significantly increased in adrenals (P < 0.05). Our findings indicate that the effects of heat challenge on the maternal ovary in the quail are mediated through the adrenal function.