Tao Sun1, Han Liu2, Yutong Cheng1, Lixiao Yan3, Chayakrit Krittanawong4, Shihong Li1, Wang Qian1, Wang Su1, Xuanzu Chen1, Xuejian Hou1, Hongju Zhang5. 1. Division of Cardiology, Anzhen Hospital Capital Medical University, Beijing, China. 2. Department of Pharmacy, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. 3. Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing, China. 4. Department of Internal Medicine, Icahn School of Medicine at Mount Sinai, New York, New York. 5. Department of Echocardiography, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Abstract
OBJECTIVE: This study was designed to explore the protective effect of 2,3,5,4'-tetrahydroxystilbene-2-O-β-d-glucoside (TSG) against ischemia/reperfusion (I/R) injury-induced cardiomyocytes apoptosis. METHODS: The H9c2 cell I/R injury model was induced by simultaneous shortage of nutrients and oxygen. TSG administration (0.10, 0.25, and 0.50 mM) was performed before and during I/R stimulation. Cell apoptosis was evaluated using terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Expression of cell-related proteins was detected to assess the effect of TSG on cell apoptosis. RESULTS: I/R injury induced significant cell apoptosis. Significantly decreased Bcl-2 and increased Bax, caspase-3, and p-Akt expression ( P < 0.01) was detected in the cell model of I/R injury. In contrast, TSG administration eliminated all the changes induced by I/R injury in a dose-dependent manner. Compared with the H9c2 cell model of I/R injury, the H9c2 cells treated with 0.50 mM TSG showed the lowest cell apoptosis percentage, the highest expression of Bcl-2, and the lowest expression of Bax, caspase-3, and p-Akt ( P < 0.01). CONCLUSION: We confirmed that the protective effect of TSG against I/R injury-induced cell apoptosis in H9c2 in vitro was associated with the Bcl-2/Bax ratio, caspase-3, and Akt activation.
OBJECTIVE: This study was designed to explore the protective effect of 2,3,5,4'-tetrahydroxystilbene-2-O-β-d-glucoside (TSG) against ischemia/reperfusion (I/R) injury-induced cardiomyocytes apoptosis. METHODS: The H9c2 cell I/R injury model was induced by simultaneous shortage of nutrients and oxygen. TSG administration (0.10, 0.25, and 0.50 mM) was performed before and during I/R stimulation. Cell apoptosis was evaluated using terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Expression of cell-related proteins was detected to assess the effect of TSG on cell apoptosis. RESULTS: I/R injury induced significant cell apoptosis. Significantly decreased Bcl-2 and increased Bax, caspase-3, and p-Akt expression ( P < 0.01) was detected in the cell model of I/R injury. In contrast, TSG administration eliminated all the changes induced by I/R injury in a dose-dependent manner. Compared with the H9c2 cell model of I/R injury, the H9c2 cells treated with 0.50 mM TSG showed the lowest cell apoptosis percentage, the highest expression of Bcl-2, and the lowest expression of Bax, caspase-3, and p-Akt ( P < 0.01). CONCLUSION: We confirmed that the protective effect of TSG against I/R injury-induced cell apoptosis in H9c2 in vitro was associated with the Bcl-2/Bax ratio, caspase-3, and Akt activation.
Authors: Jian Xiong Ma; Bin Wang; Cai Fei Ding; Hai Song Li; Xue Juan Jiang; Chen Ye Wang; Jia Yu; Wang Qiang Chen Journal: Biosci Rep Date: 2020-02-28 Impact factor: 3.840