Literature DB >> 3089155

Identification of epsilon-N-mono-, epsilon-N-di-, and epsilon-N-trimethyllysine by high-performance liquid chromatography.

R Garcia, W R Hiatt, S Jasinski, P S Sypherd.   

Abstract

Dansyl derivatives of epsilon-N-mono-, epsilon-N-di-, and epsilon-N-trimethyllysine were resolved from other amino acids in proteins by the use of high-performance liquid chromatography. The system was tested with amino acid standard combinations as well as with acid-hydrolyzed proteins known to contain methylated residues. In all cases the methylated lysines were well resolved.

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Year:  1986        PMID: 3089155      PMCID: PMC239071          DOI: 10.1128/aem.51.6.1355-1357.1986

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  4 in total

Review 1.  Protein methylation: chemical, enzymological, and biological significance.

Authors:  W K Paik; S Kim
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1975

2.  Separation of amino acids by high performance liquid chromatography.

Authors:  E Bayer; E Grom; B Kalteneger; R Uhmann
Journal:  Anal Chem       Date:  1976-07       Impact factor: 6.986

3.  Identification and location of episilon-N-trimethyllysine in yeast cytochromes c.

Authors:  R J DeLange; A N Glazer; E L Smith
Journal:  J Biol Chem       Date:  1970-07-10       Impact factor: 5.157

4.  Methylation of elongation factor 1 alpha from the fungus Mucor.

Authors:  W R Hiatt; R Garcia; W C Merrick; P S Sypherd
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

  4 in total
  1 in total

1.  Serological reactivity and biochemical characterization of methylated and unmethylated forms of a recombinant protein fragment derived from outer membrane protein B of Rickettsia typhi.

Authors:  Chien-Chung Chao; Zhiwen Zhang; Hui Wang; Abdulnaser Alkhalil; Wei-Mei Ching
Journal:  Clin Vaccine Immunol       Date:  2008-02-20
  1 in total

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