Francis Cazzeli Thompson1, Mariza Akemi Matsumoto2, Claudia Cristina Biguetti3, Ana Claudia Muniz Rennó4, Leandro de Andrade Holgado1, Joel Ferreira Santiago Junior5, Marcelo Salles Munerato1, Patrícia Pinto Saraiva3. 1. Oral Biology Post-graduation Program, Sagrado Coração University - USC, Rua Irmã Arminda 10-50 - Bauru -, SP 17011-160, Brazil. 2. Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Rua José Bonifácio 1193, 16015-050 Araçatuba, SP, Brazil. Electronic address: mariza.am@hotmail.com. 3. Faculty of Medicine of Jau - Universidade do Oeste Paulista - UNOESTE, Jaú - Brazil, R. Ângelo Martins, 498 - Jardim Estadio, Jaú - SP, 17203-480, Brazil. 4. Department of Bioscience, Federal University of Sao Paulo, Campus Baixada Santista - UNIFESP, Avenida Ana Costa, 95, Santos, SP 11060-001, Brazil. 5. Oral Biology Post-graduation Program, Sagrado Coração University - USC, Rua Irmã Arminda 10-50 - Bauru -, SP 17011-160, Brazil. Electronic address: jf.santiagojunior@gmail.com.
Abstract
OBJECTIVES: To follow healing process of augmented maxillary sinus in rabbits analyzing the histological pattern of bone tissue formation, along with the osteogenic activity and vascularization using a bioactive vitroceramic in comparison to deproteinized bovine bone associated or not with autogenous bone graft. DESIGN: Forty five male adult New Zealand rabbits, 5 months of age, mean weight of 4 Kg, underwent bilateral sinus augmentation surgeries to be divided in five groups: G - (Control) particulate autogenous bone graft (AG), BO - deproteinized bovine bone, BO+G - deproteinized bovine bone + AG, BSi -vitroceramic, and BSi + G - vitroceramic +AG. After 15, 45 and 90 days, all animals were euthanized for specimen's removal to be analyzed under light microscopy, histomorphometry, and immunohistochemistry for Runx2 and VEGF labeling. RESULTS: G, BO and BO+G groups healed uneventfully, allowing the formation of mature remodeling bone at day 90, regarding the association of AG with the biomaterial. On the other hand, BSi and BSi + G groups showed an important cellular reaction and granulation/fibrous tissue formation from the first to the last period of observation. Runx-2 and VEGF immunolabeling were coherent with this result. However, histomorphometry did not reveal significant differences considering new bone formation. CONCLUSIONS: Reconstructed maxillary sinuses using Biosilicate® permitted satisfactory new bone formation in comparison to the deproteinized bovine bone and AG. However, the presence of granulation/fibrous tissue and inflammatory cells associated to the degrading biomaterial indicate that further studies should be careful performed considering the immunological aspect of this new biomaterial.
OBJECTIVES: To follow healing process of augmented maxillary sinus in rabbits analyzing the histological pattern of bone tissue formation, along with the osteogenic activity and vascularization using a bioactive vitroceramic in comparison to deproteinized bovine bone associated or not with autogenous bone graft. DESIGN: Forty five male adult New Zealand rabbits, 5 months of age, mean weight of 4 Kg, underwent bilateral sinus augmentation surgeries to be divided in five groups: G - (Control) particulate autogenous bone graft (AG), BO - deproteinized bovine bone, BO+G - deproteinized bovine bone + AG, BSi -vitroceramic, and BSi + G - vitroceramic +AG. After 15, 45 and 90 days, all animals were euthanized for specimen's removal to be analyzed under light microscopy, histomorphometry, and immunohistochemistry for Runx2 and VEGF labeling. RESULTS: G, BO and BO+G groups healed uneventfully, allowing the formation of mature remodeling bone at day 90, regarding the association of AG with the biomaterial. On the other hand, BSi and BSi + G groups showed an important cellular reaction and granulation/fibrous tissue formation from the first to the last period of observation. Runx-2 and VEGF immunolabeling were coherent with this result. However, histomorphometry did not reveal significant differences considering new bone formation. CONCLUSIONS: Reconstructed maxillary sinuses using Biosilicate® permitted satisfactory new bone formation in comparison to the deproteinized bovine bone and AG. However, the presence of granulation/fibrous tissue and inflammatory cells associated to the degrading biomaterial indicate that further studies should be careful performed considering the immunological aspect of this new biomaterial.