| Literature DB >> 30888588 |
Atit Silsirivanit1,2,3, Chatchai Phoomak1,2,3, Karuntarat Teeravirote1,2,3, Sasiprapa Wattanavises1,2,3, Wunchana Seubwai2,3,4, Charupong Saengboonmee1,3, Zhaoqi Zhan5, Jin-Ichi Inokuchi6, Akemi Suzuki6, Sopit Wongkham7,8,9.
Abstract
Alteration of glycosphingolipid (GSL) synthesis is observed in many types of cancer. In this study, we have analyzed the expression of sphingolipids and GSLs in cholangiocarcinoma (CCA) tissues and adjacent normal liver tissues. Neutral lipids were extracted from tissue samples using mild-alkaline treatment method followed by TLC and LC-MS analysis. The expression of ceramides, hexosylceramides (HexCer), and lactosylceramides (LacCer) was altered in CCA tissues, 61.1% (11/18) of them showing an increase whereas 38.9% (7/18) showing a decrease, compared with the adjacent normal tissue. Cers and GSLs containing 2-hydroxylated fatty acids except one LacCer molecular species were overexpressed in CCA tissues, and the increase of LacCer (d18:1-h23:0) was correlated with shorter survival of CCA patients, suggesting the involvement of GSL synthesis and fatty acid hydroxylation in progression of CCA. Taken together, we have demonstrated in this study the increase of GSL synthesis and fatty hydroxylation in CCA, which probably be used as a target for CCA treatment.Entities:
Keywords: Bile ducts; Biomarker; Glycan; Glycolipid; Glycosylation
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Year: 2019 PMID： 30888588 DOI： 10.1007/s10719-019-09864-4
Source DB: PubMed Journal: Glycoconj J ISSN： 0282-0080 Impact factor: 2.916