Xuan Yu1, Cong Gao2, Caifeng Dai1, Fang Yang1, Xiaohui Deng3. 1. Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China. 2. Department of Burns Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China. 3. Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China. Electronic address: dxh250012@126.com.
Abstract
RESEARCH QUESTION: The aim of this study was to compare expression of hypoxia-inducible factor 1-alpha (HIF-1α), angiogenesis and apoptosis in endometrial tissue near the implantation window of women with recurrent implantation failure (RIF) and in fertile control women, and to describe possible mechanisms of endometrial injury. DESIGN: A controlled clinical study was conducted. Endometrial tissue specimens were obtained from 20 women undergoing IVF who had had at least three previous failed treatment cycles; normal endometrial specimens were obtained from 10 fertile control women. RESULTS: HIF-1α expression was down-regulated in the endometrium of women with RIF compared with that of control women. In addition, micro-vessel density (MVD) was much lower in the endometrium of women with RIF than in that of the control women. Apoptosis was significantly reduced in the endometrium of the RIF group compared with the control group. Endometrial injury increased HIF-1α expression and MVD in endometrial samples of the RIF group, but apoptosis was not significantly altered. CONCLUSIONS: HIF-1α expression, MVD and endometrial apoptosis were reduced in the peri-implantation endometrium of women with RIF. This suggests that altered endometrial HIF-1α expression and angiogenesis may contribute to implantation failure.
RESEARCH QUESTION: The aim of this study was to compare expression of hypoxia-inducible factor 1-alpha (HIF-1α), angiogenesis and apoptosis in endometrial tissue near the implantation window of women with recurrent implantation failure (RIF) and in fertile control women, and to describe possible mechanisms of endometrial injury. DESIGN: A controlled clinical study was conducted. Endometrial tissue specimens were obtained from 20 women undergoing IVF who had had at least three previous failed treatment cycles; normal endometrial specimens were obtained from 10 fertile control women. RESULTS: HIF-1α expression was down-regulated in the endometrium of women with RIF compared with that of control women. In addition, micro-vessel density (MVD) was much lower in the endometrium of women with RIF than in that of the control women. Apoptosis was significantly reduced in the endometrium of the RIF group compared with the control group. Endometrial injury increased HIF-1α expression and MVD in endometrial samples of the RIF group, but apoptosis was not significantly altered. CONCLUSIONS: HIF-1α expression, MVD and endometrial apoptosis were reduced in the peri-implantation endometrium of women with RIF. This suggests that altered endometrial HIF-1α expression and angiogenesis may contribute to implantation failure.