Gulcan Sahal1, Behzad Nasseri2,3, Aliakbar Ebrahimi4, Isil Seyis Bilkay5. 1. Biotechnology Division, Biology Department, Faculty of Sciences, Hacettepe University, 06800, Beytepe, Ankara, Turkey. gulcanozbakir@gmail.com. 2. Chemical Engineering and Applied Chemistry Department, Atilim University, 06830, Ankara, Turkey. 3. Chemical Engineering Department and Bioengineering Division, Hacettepe University, 06800, Beytepe, Ankara, Turkey. 4. Nanotechnology and Nanomedicine Division, Hacettepe University, 06800, Beytepe, Ankara, Turkey. 5. Biotechnology Division, Biology Department, Faculty of Sciences, Hacettepe University, 06800, Beytepe, Ankara, Turkey.
Abstract
OBJECTIVE: As an approach to prevent biofilm infections caused by Candida tropicalis on various surfaces, determination of effect of biodegradable polycaprolactone nanofibers (PCLNFs) with different concentrations of two different essential oils were tested in this study. RESULTS: Both of the tested essential oils exhibited antifungal effect (minimal inhibitory concentration; 0.25-0.49 µL/mL, minimal fungicidal concentration; 0.25-0.49 µL/mL, depending on the C. tropicalis strain) (Zone of inhibition caused by 500 μL/mL concentration of oils; 28-56 mm). 0, 2, 4% clove oil PCLNFs and 0, 2, 4% red thyme oil-PCLNFs were free from bead formation and uniform in diameter. Diameters of all essential oil containing PCLNFs were ranged from 760 to 1100 nm and were significantly different from 0% essential oil-PCLNF (P < 0.05). 0, 2, 4% clove oil-PCLNFs were significantly more hydrophobic compared to 8% clove oil-PCLNF (P < 0.01), whereas 0% and 2% red thyme oil-PCLNFs were significantly more hydrophobic compared to 4% and 8% red thyme oil PCLNFs (P < 0.01). Highest amount of biofilm inhibition was observed by 4% clove oil-PCLNF and by 4% red thyme oil-PCLNF. CONCLUSIONS: Clove and red thyme oils may be used not only as antifungals but also as biofilm inhibitive agents on surfaces of biomaterials that are frequently contaminated by C. tropicalis, when they are incorporated into PCLNFs.
OBJECTIVE: As an approach to prevent biofilm infections caused by Candida tropicalis on various surfaces, determination of effect of biodegradable polycaprolactone nanofibers (PCLNFs) with different concentrations of two different essential oils were tested in this study. RESULTS: Both of the tested essential oils exhibited antifungal effect (minimal inhibitory concentration; 0.25-0.49 µL/mL, minimal fungicidal concentration; 0.25-0.49 µL/mL, depending on the C. tropicalis strain) (Zone of inhibition caused by 500 μL/mL concentration of oils; 28-56 mm). 0, 2, 4% cloveoil PCLNFs and 0, 2, 4% redthymeoil-PCLNFs were free from bead formation and uniform in diameter. Diameters of all essential oil containing PCLNFs were ranged from 760 to 1100 nm and were significantly different from 0% essential oil-PCLNF (P < 0.05). 0, 2, 4% cloveoil-PCLNFs were significantly more hydrophobic compared to 8% cloveoil-PCLNF (P < 0.01), whereas 0% and 2% redthymeoil-PCLNFs were significantly more hydrophobic compared to 4% and 8% redthymeoil PCLNFs (P < 0.01). Highest amount of biofilm inhibition was observed by 4% cloveoil-PCLNF and by 4% redthymeoil-PCLNF. CONCLUSIONS:Clove and redthyme oils may be used not only as antifungals but also as biofilm inhibitive agents on surfaces of biomaterials that are frequently contaminated by C. tropicalis, when they are incorporated into PCLNFs.
Authors: Joana M Domingues; Marta O Teixeira; Marta A Teixeira; David Freitas; Samira F da Silva; Shafagh D Tohidi; Rui D V Fernandes; Jorge Padrão; Andrea Zille; Carla Silva; Joana C Antunes; Helena P Felgueiras Journal: Pharmaceutics Date: 2022-01-27 Impact factor: 6.321
Authors: Gulcan Sahal; Herman J Woerdenbag; Wouter L J Hinrichs; Anita Visser; Henny C van der Mei; Isil Seyis Bilkay Journal: Bio Protoc Date: 2021-03-05