Literature DB >> 3087609

Effect of lipopolysaccharide mutations and temperature on plasmid transformation efficiency in Pseudomonas aeruginosa.

D Berry, A M Kropinski.   

Abstract

We have isolated, and characterized electrophoretically, two new lipopolysaccharide-defective (rough) mutants of Pseudomonas aeruginosa strain PAO. These strains, AK1401 and AK1414, together with two previously characterized isolates, AK1012 and AK1282, were used as recipients in transformation experiments with plasmid pR01614 DNA. The roughest mutant, AK1282, was not transformable, while the transformation efficiency of AK1012, and to a lesser extent the wild-type strain, was dependent upon the growth temperature. The two new isolates which are less rough than AK1012 were transformed at a frequency equivalent to that of the wild type-strain.

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Year:  1986        PMID： 3087609     DOI： 10.1139/m86-082

Source DB:  PubMed          Journal:  Can J Microbiol        ISSN： 0008-4166            Impact factor:   2.419


  33 in total

1.  The pvc gene cluster of Pseudomonas aeruginosa: role in synthesis of the pyoverdine chromophore and regulation by PtxR and PvdS.

Authors:  A Stintzi; Z Johnson; M Stonehouse; U Ochsner; J M Meyer; M L Vasil; K Poole
Journal:  J Bacteriol       Date:  1999-07       Impact factor: 3.490

2.  Transduction of a plasmid containing the bacteriophage D3 cos site in Pseudomonas aeruginosa.

Authors:  R Sharp; E Gertman; M A Farinha; A M Kropinski
Journal:  J Bacteriol       Date:  1990-06       Impact factor: 3.490

3.  Common antigen lipopolysaccharide from Pseudomonas aeruginosa AK1401 as a receptor for bacteriophage A7.

Authors:  M Rivera; T R Chivers; J S Lam; E J McGroarty
Journal:  J Bacteriol       Date:  1992-04       Impact factor: 3.490

4.  Growth-dependent alterations in production of serotype-specific and common antigen lipopolysaccharides in Pseudomonas aeruginosa PAO1.

Authors:  E J McGroarty; M Rivera
Journal:  Infect Immun       Date:  1990-04       Impact factor: 3.441

5.  Visualization of Pseudomonas aeruginosa O antigens by using a protein A-dextran-colloidal gold conjugate with both immunoglobulin G and immunoglobulin M monoclonal antibodies.

Authors:  J S Lam; M Y Lam; L A MacDonald; R E Hancock
Journal:  J Bacteriol       Date:  1987-08       Impact factor: 3.490

6.  Expression of the multidrug resistance operon mexA-mexB-oprM in Pseudomonas aeruginosa: mexR encodes a regulator of operon expression.

Authors:  K Poole; K Tetro; Q Zhao; S Neshat; D E Heinrichs; N Bianco
Journal:  Antimicrob Agents Chemother       Date:  1996-09       Impact factor: 5.191

7.  Interaction of gentamicin with the A band and B band lipopolysaccharides of Pseudomonas aeruginosa and its possible lethal effect.

Authors:  J L Kadurugamuwa; J S Lam; T J Beveridge
Journal:  Antimicrob Agents Chemother       Date:  1993-04       Impact factor: 5.191

8.  Structure and regulation of the carAB operon in Pseudomonas aeruginosa and Pseudomonas stutzeri: no untranslated region exists.

Authors:  D H Kwon; C D Lu; D A Walthall; T M Brown; J E Houghton; A T Abdelal
Journal:  J Bacteriol       Date:  1994-05       Impact factor: 3.490

9.  Cloning and nucleotide sequence analysis of the ferripyoverdine receptor gene fpvA of Pseudomonas aeruginosa.

Authors:  K Poole; S Neshat; K Krebes; D E Heinrichs
Journal:  J Bacteriol       Date:  1993-08       Impact factor: 3.490

10.  Cloning and sequence analysis of a gene (pchR) encoding an AraC family activator of pyochelin and ferripyochelin receptor synthesis in Pseudomonas aeruginosa.

Authors:  D E Heinrichs; K Poole
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490
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