| Literature DB >> 30875046 |
Marie-Theres Gansauge1, Matthias Meyer2.
Abstract
Genomic library preparation from highly degraded DNA is more efficient when library molecules are prepared separately from the complementary strands of DNA fragments. We describe a protocol in which libraries are constructed from single DNA strands in a three-step procedure: single-stranded ligation of the first adapter with T4 DNA ligase in the presence of a splinter oligonucleotide, copying of the DNA strand with a proofreading polymerase, and blunt-end ligation of the second double-stranded adapter with T4 DNA ligase.Entities:
Keywords: Ancient DNA; Library preparation; NGS; Single-stranded ligation; T4 DNA ligase
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Year: 2019 PMID： 30875046 DOI： 10.1007/978-1-4939-9176-1_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745