Literature DB >> 30875041

Extraction of Highly Degraded DNA from Ancient Bones and Teeth.

Jesse Dabney1, Matthias Meyer2.   

Abstract

We provide a DNA extraction protocol optimized for the recovery of highly fragmented molecules preserved within bones and teeth. In this method, the hard tissue matrix is degraded using an EDTA/Proteinase K lysis buffer, and the DNA is purified using spin columns with silica membranes. This method efficiently recovers molecules as short as 35 base-pairs long.

Entities:  

Keywords:  Ancient DNA; DNA extraction; Degraded DNA; Paleogenomics; Silica purification

Mesh：

Substances：

Year:  2019        PMID： 30875041     DOI： 10.1007/978-1-4939-9176-1_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  Ancient genomes reveal tropical bovid species in the Tibetan Plateau contributed to the prevalence of hunting game until the late Neolithic.

Authors:  Ningbo Chen; Lele Ren; Linyao Du; Jiawen Hou; Victoria E Mullin; Duo Wu; Xueye Zhao; Chunmei Li; Jiahui Huang; Xuebin Qi; Marco Rosario Capodiferro; Alessandro Achilli; Chuzhao Lei; Fahu Chen; Bing Su; Guanghui Dong; Xiaoming Zhang
Journal:  Proc Natl Acad Sci U S A       Date:  2020-10-19       Impact factor: 11.205

Review 2.  Genomic approaches to trace the history of human brain evolution with an emerging opportunity for transposon profiling of ancient humans.

Authors:  Yilan Wang; Boxun Zhao; Jaejoon Choi; Eunjung Alice Lee
Journal:  Mob DNA       Date:  2021-10-18

Review 3.  Solid-phase silica-based extraction leads to underestimation of residual DNA in decellularized tissues.

Authors:  Tara C Schmitz; Aysegul Dede Eren; Janne Spierings; Jan de Boer; Keita Ito; Jasper Foolen
Journal:  Xenotransplantation       Date:  2020-09-15       Impact factor: 3.788

4.  Extreme dispersal or human-transport? The enigmatic case of an extralimital freshwater occurrence of a Southern elephant seal from Indiana.

Authors:  Ana M Valenzuela-Toro; Maria H Zicos; Nicholas D Pyenson
Journal:  PeerJ       Date:  2020-09-02       Impact factor: 2.984
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.