Julie A MacDonald1, Yasushi Takai2, Osamu Ishihara2, Hiroyuki Seki2, Dori C Woods1, Jonathan L Tilly3. 1. Laboratory of Aging and Infertility Research, Department of Biology, Northeastern University, Boston, Massachusetts. 2. Department of Obstetrics and Gynecology, Saitama Medical Center, Saitama Medical University, Saitama, Japan. 3. Laboratory of Aging and Infertility Research, Department of Biology, Northeastern University, Boston, Massachusetts. Electronic address: j.tilly@northeastern.edu.
Abstract
OBJECTIVE: To test if ovarian microenvironmental cues affect oogonial stem cell (OSC) function in a species-specific manner. DESIGN: Animal and human study. SETTING: Research laboratory. PATIENT(S)/ANIMAL(S): Human ovarian cells obtained from cryopreserved ovarian cortical tissue of reproductive-age women, and ovarian cells and tissues from female C57BL/6 mice. INTERVENTION(S): Mouse ovarian tissue, mouse OSCs (mOSCs) and human OSCs (hOSCs) were analyzed for extracellular matrix (ECM) protein expression, and OSCs isolated from adult mouse and human ovaries were cultured in the absence or presence of ECM proteins without or with an integrin signaling inhibitor. MAIN OUTCOME MEASURE(S): Gene expression and in vitro derived (IVD) oocyte formation. RESULT(S): Culture of mOSCs on a collagen-based ECM significantly elevated the rate of differentiation of the cells into IVD oocytes. Mouse OSCs expressed many integrins, including Arg-Gly-Asp (RGD)-binding subunits, and ECM-mediated increases in mOSC differentiation were blocked by addition of integrin-antagonizing RGD peptides. In comparison, hOSCs expressed a different pattern of integrin subunits compared with mOSCs, and hOSCs were unresponsive to a collagen-based ECM; however, hOSCs exhibited increased differentiation into IVD oocytes when cultured on laminin. CONCLUSION(S): These data, along with in silico analysis of ECM protein profiles in human ovaries, indicate that ovarian ECM-based niche components function in a species-specific manner to control OSC differentiation.
OBJECTIVE: To test if ovarian microenvironmental cues affect oogonial stem cell (OSC) function in a species-specific manner. DESIGN: Animal and human study. SETTING: Research laboratory. PATIENT(S)/ANIMAL(S): Human ovarian cells obtained from cryopreserved ovarian cortical tissue of reproductive-age women, and ovarian cells and tissues from female C57BL/6 mice. INTERVENTION(S): Mouse ovarian tissue, mouse OSCs (mOSCs) and human OSCs (hOSCs) were analyzed for extracellular matrix (ECM) protein expression, and OSCs isolated from adult mouse and humanovaries were cultured in the absence or presence of ECM proteins without or with an integrin signaling inhibitor. MAIN OUTCOME MEASURE(S): Gene expression and in vitro derived (IVD) oocyte formation. RESULT(S): Culture of mOSCs on a collagen-based ECM significantly elevated the rate of differentiation of the cells into IVD oocytes. Mouse OSCs expressed many integrins, including Arg-Gly-Asp (RGD)-binding subunits, and ECM-mediated increases in mOSC differentiation were blocked by addition of integrin-antagonizing RGD peptides. In comparison, hOSCs expressed a different pattern of integrin subunits compared with mOSCs, and hOSCs were unresponsive to a collagen-based ECM; however, hOSCs exhibited increased differentiation into IVD oocytes when cultured on laminin. CONCLUSION(S): These data, along with in silico analysis of ECM protein profiles in humanovaries, indicate that ovarian ECM-based niche components function in a species-specific manner to control OSC differentiation.
Authors: Helen F Irving-Rodgers; Katja Hummitzsch; Lydia S Murdiyarso; Wendy M Bonner; Yoshikazu Sado; Yoshifumi Ninomiya; John R Couchman; Lydia M Sorokin; Raymond J Rodgers Journal: Cell Tissue Res Date: 2009-12-23 Impact factor: 5.249
Authors: Yuting Fan; Colleen L Flanagan; Margaret A Brunette; Andrea S Jones; Brendon M Baker; Sherman J Silber; Ariella Shikanov Journal: F S Sci Date: 2021-06-10
Authors: Chonthicha Satirapod; Ning Wang; Julie A MacDonald; Minghan Sun; Dori C Woods; Jonathan L Tilly Journal: Aging (Albany NY) Date: 2020-04-17 Impact factor: 5.682
Authors: Laura Bianchi; Silvia Casini; Lorenza Vantaggiato; Agata Di Noi; Alfonso Carleo; Enxhi Shaba; Alessandro Armini; Francesco Bellucci; Giovanni Furii; Luca Bini; Ilaria Caliani Journal: Int J Environ Res Public Health Date: 2022-04-05 Impact factor: 3.390
Authors: Hannah Alberico; Zoë Fleischmann; Tyler Bobbitt; Yasushi Takai; Osamu Ishihara; Hiroyuki Seki; Richard A Anderson; Evelyn E Telfer; Dori C Woods; Jonathan L Tilly Journal: Stem Cells Date: 2022-05-27 Impact factor: 5.845