Izabela G Barbosa1, Natalia P Rocha1,2, Erica L Vieira1, Mehmet A Camkurt2, Rodrigo B Huguet3, Fabio T L Guimarães4, Gustavo E de Brito-Melo4, Vanessa A Mendonça4, Moises E Bauer5, Antonio L Teixeira1,2. 1. Laboratório Interdisciplinar de Investigação Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo Balena, 190, Room 281, Belo Horizonte, MG, Brazil. 2. Neuropsychiatry Program, Department of Psychiatry and Behavioral Sciences, McGovern Medical School, The University of Texas Health Science Center at Houston, 1941 East Road, Room 3140, Houston, TX 770054, USA. 3. Instituto de Previdência dos Servidores do Estado de Minas Gerais, Alameda Ezequiel Dias, 225, Santa Efigênia, Belo Horizonte, 30130-110, Brazil. 4. Laboratório de Imunologia, Universidade Federal dos Vales Jequitinhona e Mucuri, Rodovia MGT 367 ‒ Km 583, n° 5000. Alto da Jacuba, Diamantina, 39100-000, Brazil. 5. Laboratório de Imunologia do Estresse, Escola de Ciências, Pontifícia Universidade Católica do Rio Grande do Sul, Av. Ipiranga, 6681 Prédio 12a, Porto Alegre, 90619-900, Brazil.
Abstract
OBJECTIVE: Although accumulating evidence supports the hypothesis that immune/inflammatory mechanisms are associated with the pathophysiology of bipolar disorder (BD), data about the profile of chemokines (chemotactic cytokines) and chemokine receptors are still scarce. The current study was designed to evaluate the expression of chemokine receptors on lymphocytes of patients with BD in comparison with controls. METHODS: Thirty-three patients with type I BD (N = 21 in euthymia; N = 6 in mania/hypomania; N = 6 in depression) and 22 age- and sex-matched controls were subjected to clinical evaluation and peripheral blood draw. The expression of chemokine receptors CCR3, CCR5, CXCR4, and CXCR3 on CD4+ and CD8+ lymphocytes was assessed by flow cytometry. RESULTS: Patients with BD had decreased percentage of CD4+CXCR3+ (p = 0.024), CD4+CCR3+ (p = 0.042), and CD4+CCR5+ (0.013) lymphocytes in comparison with controls. The percentage of both CD4+ and CD8+ lymphocytes expressing the chemokine receptor CXCR4 was similar in patients with BD and controls. Likewise, the percentages of CD8+CXCR3+, CD8+CCR3+, and CD8+CCR5+ lymphocytes were similar in patients with BD and controls. CONCLUSION: Our findings reinforce the hypothesis that immune pathways, especially involving CD4+ lymphocytes, are involved in the physiopathology of BD.
OBJECTIVE: Although accumulating evidence supports the hypothesis that immune/inflammatory mechanisms are associated with the pathophysiology of bipolar disorder (BD), data about the profile of chemokines (chemotactic cytokines) and chemokine receptors are still scarce. The current study was designed to evaluate the expression of chemokine receptors on lymphocytes of patients with BD in comparison with controls. METHODS: Thirty-three patients with type I BD (N = 21 in euthymia; N = 6 in mania/hypomania; N = 6 in depression) and 22 age- and sex-matched controls were subjected to clinical evaluation and peripheral blood draw. The expression of chemokine receptors CCR3, CCR5, CXCR4, and CXCR3 on CD4+ and CD8+ lymphocytes was assessed by flow cytometry. RESULTS: Patients with BD had decreased percentage of CD4+CXCR3+ (p = 0.024), CD4+CCR3+ (p = 0.042), and CD4+CCR5+ (0.013) lymphocytes in comparison with controls. The percentage of both CD4+ and CD8+ lymphocytes expressing the chemokine receptor CXCR4 was similar in patients with BD and controls. Likewise, the percentages of CD8+CXCR3+, CD8+CCR3+, and CD8+CCR5+ lymphocytes were similar in patients with BD and controls. CONCLUSION: Our findings reinforce the hypothesis that immune pathways, especially involving CD4+ lymphocytes, are involved in the physiopathology of BD.
Authors: Olivia Bauer; Vladimir M Milenkovic; Sven Hilbert; Nina Sarubin; Johannes Weigl; Lisa-Marie Bahr; Thomas C Wetter; Barbara Heckel; Christian H Wetzel; Rainer Rupprecht; Caroline Nothdurfter Journal: Neuroimmunomodulation Date: 2021-01-27 Impact factor: 2.492
Authors: Jana Freff; Lisa Bröker; Rafael Leite Dantas; Kathrin Schwarte; Judith Bühlmeier; Isabelle Kraft; Anke Hinney; Ulrike Buhlmann; Volker Arolt; Udo Dannlowski; Georg Romer; Bernhard T Baune; Johannes Hebebrand; Manuel Föcker; Judith Alferink Journal: Front Psychiatry Date: 2022-09-26 Impact factor: 5.435