| Literature DB >> 30864128 |
Ashley S Brott1, David Sychantha1, Anthony J Clarke2.
Abstract
The polysaccharides that comprise bacterial cell walls are commonly O-acetylated. This modification confers resistance to hydrolases of innate immune systems and/or controls endogenous autolytic activity. Herein, we present protocols for the compositional analysis of bacterial cell wall O-acetylation, and assays for monitoring O-acetyltransferases and O-acetylesterases. The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.Entities:
Keywords: Chitooligosaccharides; O-Acetylation; O-acetylesterase; O-acetyltransferase; Peptidoglycan
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Year: 2019 PMID： 30864128 DOI： 10.1007/978-1-4939-9154-9_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745