Xiaodong Huang1, Weiheng Wang1, Qingxi Meng1, Lei Yu1, Chunquan Fan2, Jiangming Yu1, Mintao Xue3, Xiaojian Ye4. 1. Department of Orthopaedics, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, 200003, China. 2. Department of Orthopaedic Surgery, The 175th Hospital of PLA, Orthopaedics Center of PLA, Affiliated Southeast Hospital of Xiamen University, Zhangzhou, 363000, Fujian Province, China. 3. Department of Orthopaedics, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, 200003, China. xmt1984@smmu.edu.cn. 4. Department of Orthopaedics, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, 200003, China. xjyespine@smmu.edu.cn.
Abstract
PURPOSE: Discography can increase disc degeneration, but the influence of different discography variables on the degeneration of discs has not been reported. The aim of this study was to investigate the effects of discography variables of needle diameter, type of contrast agent and volume of contrast agent on disc degeneration. METHODS: Three separate experiments examined needle diameter, and type and volume of contrast agent. Coccygeal discs (Co7-10) adult male rats were used. X-rays were used to detect the disc height degeneration index at 1, 2 and 4 weeks after the procedure. MRI was used to study the changes in the disc structure and the signal intensity of IVD 2 and 4 weeks after the procedure. Disc water content and histology were measured at 4 weeks after the procedure. RESULTS: A 21-g needle significantly increased disc degeneration when compared with the 30-g needle as detected by X-ray, MRI, disc water content and histology (P < 0.05). Two microlitres of iodine significantly decreased the disc MRI signal and water content at 4 weeks compared with the same volume of normal saline (P < 0.05). Three microlitres of iodine significantly increased disc degeneration when compared with 2 µl iodine, as detected by X-ray, MRI, disc water content and histology at 4 weeks (P < 0.05). CONCLUSION: To reduce disc degeneration after discography, it may be best to choose a smaller needle size, minimize the use of contrast agent and use non-ionic contrast agents with osmotic pressure similar to the intervertebral disc. These slides can be retrieved under Electronic Supplementary Material.
PURPOSE: Discography can increase disc degeneration, but the influence of different discography variables on the degeneration of discs has not been reported. The aim of this study was to investigate the effects of discography variables of needle diameter, type of contrast agent and volume of contrast agent on disc degeneration. METHODS: Three separate experiments examined needle diameter, and type and volume of contrast agent. Coccygeal discs (Co7-10) adult male rats were used. X-rays were used to detect the disc height degeneration index at 1, 2 and 4 weeks after the procedure. MRI was used to study the changes in the disc structure and the signal intensity of IVD 2 and 4 weeks after the procedure. Disc water content and histology were measured at 4 weeks after the procedure. RESULTS: A 21-g needle significantly increased disc degeneration when compared with the 30-g needle as detected by X-ray, MRI, disc water content and histology (P < 0.05). Two microlitres of iodine significantly decreased the disc MRI signal and water content at 4 weeks compared with the same volume of normal saline (P < 0.05). Three microlitres of iodine significantly increased disc degeneration when compared with 2 µl iodine, as detected by X-ray, MRI, disc water content and histology at 4 weeks (P < 0.05). CONCLUSION: To reduce disc degeneration after discography, it may be best to choose a smaller needle size, minimize the use of contrast agent and use non-ionic contrast agents with osmotic pressure similar to the intervertebral disc. These slides can be retrieved under Electronic Supplementary Material.
Entities:
Keywords:
Contrast agent type and volume; Disc degeneration; Discography; Needle diameter; Rat tail model
Authors: Juliane D Glaeser; Wafa Tawackoli; Derek G Ju; Jae H Yang; Linda Ea Kanim; Khosrowdad Salehi; Victoria Yu; Evan Saidara; Jean-Phillipe Vit; Zhanna Khnkoyan; Zachary NaPier; Laura S Stone; Hyun W Bae; Dmitriy Sheyn Journal: JOR Spine Date: 2020-06-22
Authors: Jesil Kasamkattil; Anna Gryadunova; Ivan Martin; Andrea Barbero; Stefan Schären; Olga Krupkova; Arne Mehrkens Journal: Int J Mol Sci Date: 2022-02-25 Impact factor: 6.208