Literature DB >> 3086258

Increased acidophilia of eosinophil granules after EDTA treatment.

C Gomez-Perretta, R Armas-Portela, J C Stockert, A Tato, J M Ferrer.   

Abstract

The acidophilic reaction of eosinophil leucocyte granules from human, pig and horse blood smears was investigated by using May-Grünwald-Giemsa staining after previous treatment with EDTA and sodium citrate solutions. The same peak at 530 nm, but absorption values considerably higher than those of controls, were found in eosinophil granules after application of chelating agents, indicating that removal of metal cations could unmask basic groups in these structures.

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Year:  1986        PMID： 3086258     DOI： 10.1007/bf01676191

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  14 in total

1.  Fluorescence reaction of leukocyte granules by morin.

Authors:  J C Stockert; O D Colman; M Cañete
Journal:  Acta Histochem Suppl       Date:  1985

Review 2.  Studies on metal ions-DNA interactions: specific behaviour of reiterative DNA sequences.

Authors:  I Sissoëff; J Grisvard; E Guillé
Journal:  Prog Biophys Mol Biol       Date:  1976       Impact factor: 3.667

3.  Mechanisms involved in the banding of chromosomes with guinacrine and Giems. II. The interaction of the dyes with the chromosomal components.

Authors:  A T Sumner; H J Evans
Journal:  Exp Cell Res       Date:  1973-09       Impact factor: 3.905

4.  A new staining procedure for electron microscopical cytology.

Authors:  W Bernhard
Journal:  J Ultrastruct Res       Date:  1969-05

Review 5.  On the nature of Romanowsky--Giemsa staining and its significance for cytochemistry and histochemistry: an overall view.

Authors:  D H Wittekind
Journal:  Histochem J       Date:  1983-10

6.  Immunocytochemical localization of two peroxisomal enzymes of lipid beta-oxidation in specific granules of rat eosinophils.

Authors:  S Yokota; W Deimann; T Hashimoto; H D Fahimi
Journal:  Histochemistry       Date:  1983

7.  On the nature of Romanowsky-Giemsa staining and the Romanowsky-Giemsa effect. I. Model experiments on the specificity of azure B-eosin Y stain as compared with other thiazine dye-eosin Y combinations.

Authors:  D H Wittekind; T Gehring
Journal:  Histochem J       Date:  1985-03

8.  Localization of lysosomal and peroxisomal enzymes in the specific granules of rat intestinal eosinophil leukocytes revealed by immunoelectron microscopic techniques.

Authors:  S Yokota; H Tsuji; K Kato
Journal:  J Histochem Cytochem       Date:  1984-03       Impact factor: 2.479

9.  ISOLATION OF GRANULES FROM EOSINOPHIL LEUCOCYTES AND STUDY OF THEIR ENZYME CONTENT.

Authors:  G T ARCHER; J G HIRSCH
Journal:  J Exp Med       Date:  1963-08-01       Impact factor: 14.307

10.  Localization of eosinophil granule major basic protein in human basophils.

Authors:  S J Ackerman; G M Kephart; T M Habermann; P R Greipp; G J Gleich
Journal:  J Exp Med       Date:  1983-09-01       Impact factor: 14.307
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  1 in total

1.  Fluorescence of eosinophil leucocyte granules induced by 1-hydroxy-3,6,8-pyrenetrisulfonate. Visualization of differences in protein isoelectric points.

Authors:  C I Trigoso; J Espada; J C Stockert
Journal:  Histochem Cell Biol       Date:  1995-07       Impact factor: 4.304
  1 in total

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