Literature DB >> 3086162

A method for the mass isolation of islets from the adult pig pancreas.

C Ricordi, E H Finke, P E Lacy.   

Abstract

A method for mass isolation of islets from the pig pancreas is described. The procedure involves the use of an enzymatic and mechanical pancreatic digestion procedure followed by filtration and separation of the islets on Ficoll gradients. A remarkably high yield of purified islets has been obtained from the pig pancreas with this procedure. The islets are morphologically intact and respond to acute stimulation with glucose in vitro.

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Year:  1986        PMID： 3086162     DOI： 10.2337/diab.35.6.649

Source DB:  PubMed          Journal:  Diabetes        ISSN： 0012-1797            Impact factor:   9.461


  13 in total

1.  Islet isolation assessment in man and large animals.

Authors:  C Ricordi; D W Gray; B J Hering; D B Kaufman; G L Warnock; N M Kneteman; S P Lake; N J London; C Socci; R Alejandro
Journal:  Acta Diabetol Lat       Date:  1990 Jul-Sep

2.  Immunohistochemical and ultrastructural study of adult porcine endocrine pancreas during the different steps of islet isolation.

Authors:  M C Vantyghem; J Kerr-Conte; F Pattou; M H Gevaert; C Hober; A Defossez; M Mazzuca; J C Beauvillain
Journal:  Histochem Cell Biol       Date:  1996-11       Impact factor: 4.304

3.  Lymphokine release during co-culture of human lympho-mononuclear cells and fresh or cultured human, porcine and bovine pancreatic islets.

Authors:  R Giannarelli; M Ferdeghini; C Arvia; R Consolini; P Marchetti; A Coppelli; C Prontera; A Legitimo; M Carmellini; F Mosca; R Navalesi
Journal:  Acta Diabetol       Date:  1996-07       Impact factor: 4.280

4.  High-performance liquid chromatography analysis of circulating insulins distinguishes between endogenous insulin production (a potential pitfall with streptozotocin diabetic rats) and islet xenograft function.

Authors:  D Chicheportiche; S Darquy; J Lepeintre; F Capron; P A Halban; G Reach
Journal:  Diabetologia       Date:  1990-08       Impact factor: 10.122

5.  Prevention of oxidative stress in porcine islet isolation.

Authors:  Philipp Stiegler; Vanessa Stadlbauer; Florian Hackl; Silvia Schaffellner; Florian Iberer; Joachim Greilberger; Dirk Strunk; Sieglinde Zelzer; Carolin Lackner; Karlheinz Tscheliessnigg
Journal:  J Artif Organs       Date:  2010-03-06       Impact factor: 1.731

6.  [In vitro and in vivo studies of isolation, transplantation and function of Langerhans islets in the swine].

Authors:  U J Hesse; J Danis; J Weyer; G Meyer; S Saad
Journal:  Langenbecks Arch Chir       Date:  1990

7.  Feeding NOD mice with pig splenocytes induces transferable mechanisms that modulate cellular and humoral xenogeneic reactions against pig spleen or islet cells.

Authors:  S You; E Gouin; P Saï
Journal:  Clin Exp Immunol       Date:  2002-03       Impact factor: 4.330

8.  Xenogeneic islet transplantation of microencapsulated porcine islets for therapy of type I diabetes: long-term normoglycemia in STZ-diabetic rats without immunosuppression.

Authors:  Thomas Meyer; Burkhard Höcht; Karin Ulrichs
Journal:  Pediatr Surg Int       Date:  2008-12       Impact factor: 1.827

9.  Human monoclonal islet cell antibodies from a patient with insulin-dependent diabetes mellitus reveal glutamate decarboxylase as the target antigen.

Authors:  W Richter; J Endl; T H Eiermann; M Brandt; R Kientsch-Engel; C Thivolet; H Jungfer; W A Scherbaum
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

Review 10.  Pig-to-nonhuman primates pancreatic islet xenotransplantation: an overview.

Authors:  Marco Marigliano; Suzanne Bertera; Maria Grupillo; Massimo Trucco; Rita Bottino
Journal:  Curr Diab Rep       Date:  2011-10       Impact factor: 4.810
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