MOTIVATION: During development of the mammalian embryo, histone modification H3K4me3 plays an important role in regulating gene expression and exhibits extensive reprograming on the parental genomes. In addition to these dramatic epigenetic changes, certain unchanging regulatory elements are also essential for embryonic development. RESULTS: Using large-scale H3K4me3 chromatin immunoprecipitation sequencing data, we identified a form of H3K4me3 that was present during all eight stages of the mouse embryo before implantation. This 'stable H3K4me3' was highly accessible and much longer than normal H3K4me3. Moreover, most of the stable H3K4me3 was in the promoter region and was enriched in higher chromatin architecture. Using in-depth analysis, we demonstrated that stable H3K4me3 was related to higher gene expression levels and transcriptional initiation during embryonic development. Furthermore, stable H3K4me3 was much more active in blood tumor cells than in normal blood cells, suggesting a potential mechanism of cancer progression. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
MOTIVATION: During development of the mammalian embryo, histone modification H3K4me3 plays an important role in regulating gene expression and exhibits extensive reprograming on the parental genomes. In addition to these dramatic epigenetic changes, certain unchanging regulatory elements are also essential for embryonic development. RESULTS: Using large-scale H3K4me3 chromatin immunoprecipitation sequencing data, we identified a form of H3K4me3 that was present during all eight stages of the mouse embryo before implantation. This 'stable H3K4me3' was highly accessible and much longer than normal H3K4me3. Moreover, most of the stable H3K4me3 was in the promoter region and was enriched in higher chromatin architecture. Using in-depth analysis, we demonstrated that stable H3K4me3 was related to higher gene expression levels and transcriptional initiation during embryonic development. Furthermore, stable H3K4me3 was much more active in blood tumor cells than in normal blood cells, suggesting a potential mechanism of cancer progression. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
Authors: Maxine G B Tran; Becky A S Bibby; Lingjian Yang; Franklin Lo; Anne Y Warren; Deepa Shukla; Michelle Osborne; James Hadfield; Thomas Carroll; Rory Stark; Helen Scott; Antonio Ramos-Montoya; Charlie Massie; Patrick Maxwell; Catharine M L West; Ian G Mills; David E Neal Journal: BMC Cancer Date: 2020-05-25 Impact factor: 4.430
Authors: Anton O Chugunov; Nadezhda A Potapova; Natalia S Klimenko; Victor V Tatarskiy; Sofia G Georgieva; Nataliya V Soshnikova Journal: Int J Mol Sci Date: 2021-10-15 Impact factor: 5.923