Comparison of the efficacy of intradermal injected tranexamic acid vs hydroquinone cream in the treatment of melasma.

BACKGROUND: Melasma is a common benign acquired pigmentary dermatosis due to a disorder in the function of the melanogenesis process. Although several treatments are currently used, it remains a great challenge. AIM: The aim of this study is to compare the efficacy of intradermal injected tranexamic acid (TA) vs hydroquinone (HQ) cream in the treatment of melasma.
MATERIALS AND METHODS: In this prospective split face controlled clinical trial, 49 patients were randomly divided into two groups of A (24 persons) and B (25 persons). Patients received TA intradermal injections every 2 weeks on the right side of the face with a concentration of 4 mg/ mL in group A and a concentration of 10 mg/mL in group B. The left side in both groups was treated twice daily with topical 4% HQ cream, and treatment continued for 12 weeks in both groups. Melasma Area and Severity Index (MASI) scores were measured for each side of the face at baseline and at weeks 4, 8, 12, and 24. SPSS, version 22, P<0.05, was used for data analysis.
RESULTS: Forty-one patients (21 in group A and 20 in group B) completed the study. The MASI score in the 12th week significantly decreased compared to the baseline for group A, group B, and HQ cream. However, no statistically significant difference was observed between the MASI score of patients in groups A and B. Also, the comparison of TA at the concentration of 4 mg/ mL compared to the 4% HQ cream showed that the MASI scores in the eighth week (P=0.02) and the 12th week (P=0.02) were significantly less in the HQ group. However, no significant difference was observed between the MASI score changes in Group B (10 mg/mL) and the 4% HQ group. Also, patients in group A had higher satisfaction than patients in group B (P=0.001).
CONCLUSION: Injection of TA intradermally can be an effective treatment for melasma.

Introduction

Melasma is a common benign acquired pigmentary dermatosis due to a disorder in the function of the melanogenesis process. Melasma is often found as light to dark-brown patches and macules with irregular margins on the face symmetrically.1 Young women are often diagnosed with this disorder, and it occurs during pregnancy and hormonal changes in the ovaries.2 The main cause and pathogenicity of melasma is not yet clear, but it seems that the factors of genetic background, pregnancy, oral contraceptive pills, sunlight, ovarian tumors, hormone replacement therapy (HRT), and anticonvulsant drugs and steroids are involved in the onset of this disorder.3

In general, the treatment of melasma inhibits the production of pigment and the transfer of melanosomes into keratinocytes. Hydroquinone (HQ) cream acts as a standard treatment for various types of skin hyperpigmentation through inhibition of the enzyme affecting tyrosine oxidation (tyrosinase). Also, it can inhibit DNA and RNA synthesis reversibly and may also affect the production of melanosome.4 Other treatment modalities include: azelaic acid, 4-n-butylresorcinol and arbutin (tyrosinase inhibitor), kojic acid and ascorbic acid (interact with copper), retinoids (exfoliative agent), and corticosteroids (reduce underlying erythema).5,6 Furthermore, combination therapies including hydroquinone and retinoid and corticosteroids are used as the most effective treatments for melasma. Other therapeutic measures, such as chemical peeling and laser, are used for the treatment of resistant melisma.5–7 Possible side-effects of these treatments include: erythema, irritation, sensitivity to sunlight, dryness, skin irritation, exogenous ochronosis, desquamation, atrophy, telangiectasia, hypertrichosis, and exanthema.7,8

Recently, trans-4-(Aminomethyl)cyclohexanecarboxylic acid, or tranexamic acid (TA), has been proposed as a new treatment for melasma. In 1979, Nijo Sadako accidentally discovered its effect during treatment of a patient with chronic urticaria.9 TA is a plasmin inhibitor, which is also considered a fibrinolysis inhibitor and can be used to reduce bleeding. TA is a synthetic derivative of the amino acid lysine and exerts its antifibrinolytic effect through the reversible blockade of lysine-binding sites on plasminogen molecules. It leads to the inhibition of the plasminogen activator from plasminogen transformation into plasmin. Plasminogen is also found in the basal layer of the human epidermis.10 The main mechanism of the hypopigmentant effects of TA is due to its antiplasmin activity.11 In addition, TA is similar to tyrosine in a portion of its structure, which can inhibit tyrosinase competitively.12 Also, Plasmin transforms the vascular endothelial growth factor (VEGF) into a diffusing form, and histological examination showed that TA plays an important role in the reduction of erythema and vascularities and the number of mast cell in the dermis.13,14 Various forms of TA are used orally, topically, and as a microinjection for the treatment of melasma. The amount of TA used to treat melasma is much less than the amount required for its antifibrinolytics effects.15

The present study aimed to use two concentrations of TA, 4 and 10 mg/mL, and compare them with standard treatment (4% HQ cream).

Materials and methods

Study type and population

This prospective split face controlled clinical trial, from September 2017 to May 2018, included patients who were referred to the Dermatology Clinic of Imam Hospital of Ahvaz for the treatment of melasma and were willing to cooperate, women aged 18–50 years with bilateral symmetric melasma, who signed the consent form of the study, and were divided randomly in the basis of block permutation (four blocks) into two groups of A and B. It should also be noted that the exclusion criteria included pregnancy, lactation, oral contraceptive pills over the past 12 months, and during this study, any history of coagulation disorder and thrombotic problems, the use of anti-coagulant and anticonvulsant (phenytoin) drugs, sensitivity to the drug studied, the history of melasma treatment during the past month, herpes simplex lesions on the face, and ultimately patients with facial warts. This study was approved by the local Ethics committee of Ahvaz Jundishapur University of Medical Science, in accordance with the Declaration of Helsinki (code:IR.AJUMS.REC.1396.1091). All the patients signed the written informed consent form prior to initiation and for the images to be published.

Implementation of this study

During the first visit, each patient was examined by a Wood’s lamp, and the type of melasma (epidermal, dermal, mixed) was determined. Also, if the lesion of the patient is exacerbated by the Wood’s lamp, it is epidermal and otherwise dermal. If one part of the lesion was exacerbated and one part was unclear, it was considered as mixed. Then, the demographic characteristics of the patient, including age, sex, family history, precipitating factors, duration of melasma, previous treatments, skin phenotype, type of melasma, and affected areas, were recorded.

After taking a local anesthetic xyla-p cream (Tehran Shimi Pharmaceutical Co., Tehrean, Iran) on the right half of the face and dressing it for 1 hour, an intradermal injection of the TA (Caspian Tamin Pharmaceutical Co. Rasht, Iran) was performed using the insulin syringe and at intervals of 1 cm. The right side of the face was injected intradermally with a concentration of 4 mg/mL in group A and 10 mg/mL in group B. Injection was done every 2 weeks. Also, the left side of the face of the patients in both groups was treated twice daily with 4% HQ cream (Eldoquin Forte, ICN Company, Costa Mesa, California, USA) and all patients were recommended to use a sunscreen (with no pigment). The duration of treatment was 12 weeks.

Preparation method and injection of tranexamic acid

Tranexamic acid is available as a 5 mL vial containing 500 mg. An insulin syringe was used, with a volume of 1 mL, containing 0.04 mL (4 units of insulin) of TA and the remainder being normal saline; thus each insulin syringe contains 4 mg TA. In order to prepare the drug at a concentration of 10 mg, 0.1 mL (10 units of insulin) of the TA was required to reach a volume of 1 mL with normal saline.

Follow-up

Patients follow-up was done by measuring the Melasma Area and Severity Index (MASI) score and evaluation of side-effects every 4 weeks (weeks 0, 4, 8, and 12). MASI score was measured according to the standard guidelines (Figure 1). After completing treatment, patients were followed up 12 weeks later in order to evaluate the recurrence of melasma. Also, a general evaluation was done by the Dynamic Physician Global Assessment (Dynamic PGA) according to reduction in MASI score at the end of the treatment.

Figure 1

Modified MASI scoring

Abbreviation: MASI, Melasma Area and Severity Index.

Statistical methods of the analysis of results

The one-way analysis of variance (ANOVA) and Tukey’s test were used in order to compare the mean of quantitative variables in three groups. Kruskal-Wallis was used to compare the rank qualitative variables, and the square method was used to compare the nominal qualitative variables. T-pair test was also used before and after the test. Covariance analysis was also used in the case of the presence of an intervening variable. SPSS version 22, P<0.05, was used and a significant level of 0.05 was considered.

Results

Demographic and pre-intervention results

Forty-one female patient completed the study. Eight patients discontinued the study (three from group A and five from group B); one patient developed urticaria upon TA injections, two patients because of the distance from the clinic, three because of disaffection from treatment, and no reason was stated by two patients. The mean age of the patients was 37.70±6.90 years, and no significant difference was observed between the two groups (P=0.28). The majority of patients (46.3%) had Skin Type III, and the type of melasma among the majority of patients was epidermal (63.4%). Patients characterization in two groups are summarized in Table 1. No statistically significant difference was observed between them, except the mean duration of the disease.

Table 1

Patients characterization in two groups

Variables		Group		P-value
		Group A (%)	Group B (%)
Skin type	2	1 (4.8)	3 (15)	0.73
	3	10 (47.6)	9 (45)
	4	9 (42.9)	7 (35)
	5	1 (4.8)	1 (5)
Melasma type	Mixed	6 (28.6)	8 (40)	0.39
	Epidermal	15 (71.4)	11 (55)
	Dermal	0 (0)	1 (5)
Area affected	Central	0 (0)	2 (10)	0.16
	Malar	15 (71.4)	13 (65)
	Central malar	6 (28.6)	3 (15)
	Malar mandibular	0 (0)	2 (10)
Family history	Yes	11 (52.4)	11 (55)	0.86
	No	10 (47.6)	9 (45)
Predisposing factor	Pregnancy UV	15 (71.4)	15 (75)	0.79
	UV	6 (28.6)	5 (25)
Previous treatment	Yes	12 (57.1)	12 (60)	0.85
	No	9 (42.9)	8 (40)

Results of effectiveness of treatment with MASI score and dynamic physician global assessment

Comparison of the mean MASI at baseline in group A (4 mg/mL) and group B (10 mg/mL) shows that no statistically significant difference was observed between them. Comparison of mean MASI score in group A compared to 4% HQ cream showed that no statistically significant difference was observed between the mean MASI score at baseline (P=0.62) and the 4th week (P=0.34), but in the 8th week (P=0.02) and the 12th week (P=0.02), it was significantly less in the HQ cream 4% group. The mean MASI score in group B compared to the 4% HQ cream group showed that no statistically significant difference was observed in the mean MASI scores (Figure 2).

Figure 2

MASI score in two groups by right and left side results.

Abbreviation: MASI, Melasma Area and Severity Index

Also, the evaluation of reduction in MASI score by Physician Global Assessment showed that the comparison between groups A and B did not have a statistically signifi-cant difference (P=0.99). However, comparison of Dynamic PGA in group A with the 4% HQ cream shows PGA of the HQ group was significantly better than group A (P=0.031). Also, the comparison of Dynamic PGA in patients in group B with the 4% HQ cream group did not show a statistically significant difference (P=0.98) (Table 2).

Table 2

Dynamic PGA in two groups by right and left side results

PGA	Excellent		Good		Fair		Poor		P-value	P-value
Groups	Number	Percent	Number	Percent	Number	Percent	Number	Percent
Group A	1	4.8	4	19.0	3	14.3	13	61.9	0.031	0.99*
Group A left	3	14.3	5	23.8	6	28.6	7	33.3
Group B	1	5.0	4	20.0	3	15.0	12	60.0	0.98
Group B left	1	5.0	5	25.0	3	15.0	11	55.0

Notes:

P-value between groups A and B. Excellent (>75% reduction in MASI). Good (51–75% reduction in MASI). Fair (26–50% reduction in MASI). Poor (0–25% reduction in MASI).

Abbreviations: MASI, Melasma Area and Severity Index; PGA, Physician Global Assessment.

Satisfaction rate of patients

Finally, patients’ satisfaction rate data showed that patients in group A had a higher satisfaction rate than group B patients (P=0.001), and patients in the hydroquinone group had a higher satisfaction rate than group A, but no statistically significant difference was observed (P=0.076). Finally, no statistically significant difference was observed between the satisfaction rate of group B compared to 4% HQ cream (P=0.82) (Table 3).

Table 3

Satisfaction in two groups by right and left side results

Satisfaction	Relative		No		Complete		P-value	P-value
Groups	number	percent	number	percent	number	percent
Group A	13	61.9	7	33.3	1	4.8	0.07	<0.001*
Group A left	18	85.7	3	14.3	0	0.0
Group B	6	30.0	10	50.0	4	20.0	0.82
Group B left	9	45.0	8	40.0	3	15.0

Note:

P-value between groups A and B.

Adverse effects and relapse

All patients experienced injection site burning pain, in spite of local anesthesia; one patient reported urticaria, who discontinued treatment. No adverse effect was seen in the HQ group.

Follow-up was done at 24 weeks, but only 19 patients (11 from A and 8 from B) returned. Six patients from group A and two from Group B relapsed.

Discussion and conclusion

Melasma is a common benign acquired dermatosis found by hyperpigmented macules or patches, most commonly on the face symmetrically.1 Studies have shown that melasma causes cosmetic disfigurement and leads to psychosocial distress.16

Treatment modalities for melasma include use of antisolar creams, hydroquinone, triple combination topicals, peeling, and laser.17 TA has anti-plasmin activity, and this effect makes it a candidate for treatment of melasma, because plasmin has melanogenic and angiogenic properties. It has recently been discovered that the caliber and count of vascularity and expression of VEGF in melasma lesions increased, thereby showing that TA may clinically improve melasma by two mechanisms. In two review of the literature by Perper et al15 and Kim et al18 TA was indicated to be becoming a choice in treatment of melasma. However, there are scant studies that have assessed intradermal microinjection of TA as an effective and safe method.19–21 Previous studies had some disadvantages, mainly including lack of a control group. In 2006, Lee et al,19 during a prospective study, investigated the effect of localized TA intradermal microinjection (4 mg/mL) on 100 women with melasma. About 85 patients completed the study, and a statistically significant decrease was observed in MASI at 8 and 12 weeks. In another study in 2009, Steiner et al20 compared the effects of topical TA 3% with intradermal injection of TA (4 mg/mL) in 18 women with melasma. Group A used 3% TA twice a day and injection with TA (4 mg/mL) weekly for 12 weeks was performed for group B. Seventeen patients completed the study. According to MASI score and calorimetric evaluation, both groups improved significantly, and no significant difference was observed between them. In 2013, during a prospective study, Budamakuntla et al21 divided 60 patients into two groups. A group was under TA microinjection (4 mg/mL) and another group under TA microneedling (4 mg/mL) for 3 months (0, 4, and 8) and followed-up for 3 months. In the microinjection group, 35.72% improvement was observed in MASI score, compared with 44.41% in the microneedling. Improvement in the microneedling group was better than the microinjection group. Elfar and El-Maghraby,22 in their study in 2015, treated 60 women in different ways to compare the effects of intradermal injection of TA (20 subjects) and silymarin cream as a strong antioxidant (20) and 50% glycolic acid peeling (20). TA (4 mg/mL) was injected intradermally at intervals of 1 cm weekly for 12 weeks. Intradermal injection of tranexamic acid significantly reduced the MASI score from the baseline, but its effect was less than the silymarin cream and glycolic acid peeling. Sharma et al,23 during a study that ran during a period in in 2016, investigated 100 patients (92 women and eight men) and divided them into two groups. One group was treated with oral 250 mg tranexamic acid twice a day and the other group was injected intradermally with TA 4 mg/mL every 4 weeks. The treatment period was 3 months. Mean reduction of MASI in the 12th week was 77.96 in the oral group and 79.00 in the injection group, so no significant difference was observed between them. In another prospective comparative study in 2018, Shetty and Shetty24 divided 40 patients into two group. Group A were treated with intradermal injection of TA (4 mg/mL) once at 3 week intervals (0, 3, 6, 9, and 12 weeks) for 12 weeks and group B were treated with oral TA 250 mg twice a day for 12 weeks. According to MASI score reduction, intradermal injection of TA has a higher clinical improvement (35.6%) compared to oral TA (21.7%). In our study, the MASI score in the 12th week significantly decreased compared to the baseline for group A, group B, and HQ cream. However, no statistically significant difference was observed between the MASI score in groups A and B. Also, 4 mg/mL TA compared to the 4% HQ cream showed that the MASI score in the eighth week (P=0.02) and the 12th week (P=0.02) was significantly less in the HQ cream group. However, no significant difference was observed between the MASI score in Group B (10 mg/mL) and the 4% HQ cream group. Dynamic PGA evaluation showed that no statistically significant difference was observed between PGA in both groups A and B (P=0.99). However, comparison between group A to HQ indicated that PGA of the HQ group was significantly better than group A (P=0.031). Also, patients in group A had a higher satisfaction than patients in group B (P=0.001)

Also, the review of other studies in the research community in Iran is consistent with the results of this study, for example, during a study in 2016, Saki et al25 investigated 37 female patients with melasma, the right side of their faces was treated with 20 mg/mL TA injection monthly and the left side of their faces was treated with HQ cream 2% for 3 months. The melanin and erythema index was measured by a calorimetric device called Dermacch during the 4th, 8th, and 20th weeks. No significant difference was observed in the reduction of melanin index at the end of the follow-up period in both groups. As in the present study, tranexamic acid has no significant effect over the hydroquinone cream (Figures 3 and 4).

Figure 3

Before treatment with 4 mg/mL TA (A), after treatment with 4 mg/mL TA (B), before treatment with HQ (C), and after treatment with HQ (D).

Figure 4

Before treatment with 10 mg/mL TA (A), after treatment with 10 mg/mL TA (B), before treatment with HQ (C), and after treatment with HQ (D).

Intradermal injection of TA seems to be a safe treatment for melisma. Dose of TA and duration and interval of administration has been different in the previous study, but they all reported beneficial effects. The advantages of this study were the presence of the hydroquinone as the control group and higher concentration of TA; however, no difference was observed between 4 mg/mL and 10 mg/mL. Furthermore, compared to Sharma et al’s23 study, mean reduction of MASI score in our study was lower. This may be due to many factors such as genetics, severity, duration and type of melasma, study design, etc.

The limitations of this study were the small number of participants, lack of blindness for the investigator, the inadequate duration of treatment and follow-up, and use of the MASI score, which is a subjective assessment method.

We suggest organizing studies to accentuate the optimum dose and duration of treatment with intradermal TA injection alone or in combination with other lightening agents.

Data sharing statement

Participants data, including demographic data and MASI scores, are available on request from the corresponding author, or can be obtained via http://www.IRCT.ir for up to 2 years after publication.