Literature DB >> 3085543

A fluorometric assay for biotinidase.

H Ebrahim, K Dakshinamurti.   

Abstract

An assay for biotinidase using biocytin, the natural substrate, is described. The fluorometric procedure uses 1,2-diacetylbenzene which reacts selectively with lysine allowing its direct determination in mixture with biocytin. We have examined the applicability of the assay using human serum biotinidase.

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Year:  1986        PMID： 3085543     DOI： 10.1016/0003-2697(86)90527-0

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Biotinidase activity in patients with phenylketonuria.

Authors:  K H Schulpis; E Papakonstantinou; S Kalogirou
Journal:  J Inherit Metab Dis       Date:  1995       Impact factor: 4.982

2.  Co-purification of human serum lipoamidase and biotinidase: evidence that the two enzyme activities are due to the same enzyme protein.

Authors:  L Nilsson; B Kågedal
Journal:  Biochem J       Date:  1993-04-15       Impact factor: 3.857

3.  Comparison of Spectrophotometric and Fluorimetric Methods in Evaluation of Biotinidase Deficiency.

Authors:  Sevgin Özlem Işeri-Erten; Zeliha Günnur Dikmen; Nuriye Nuray Ulusu
Journal:  J Med Biochem       Date:  2016-05-09       Impact factor: 3.402
  3 in total

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