Yanqi Zhang1, Limin Zhang2,3, Guoyan Yin1, Wenjuan Ma4, Jiao Li1,5, Zhongxing Zhou1,5, Feng Gao6,7. 1. School of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, 300072, China. 2. School of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, 300072, China. zhanglm@tju.edu.cn. 3. Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin, 300072, China. zhanglm@tju.edu.cn. 4. Cancer Institute and Hospital, Tianjin Medical University, Tianjin, 300060, China. 5. Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin, 300072, China. 6. School of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, 300072, China. gaofeng@tju.edu.cn. 7. Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin, 300072, China. gaofeng@tju.edu.cn.
Abstract
PURPOSE: The purpose of this study was to show a systematic strategy for assessing the pharmacokinetics of indocyanine green (ICG)-loaded nanoparticles in the tumor tissue based on a dynamic diffuse fluorescence tomography (DFT) system. PROCEDURES: Twelve-seven-week-old male Balb/c nude mice bearing HepG2/ADR hepatocellular carcinoma were randomly divided into four groups (n = 3 per group). Four hundred microliters of three types of ICG-loaded nanoparticles (content of ICG: 50 μg/ml) and free ICG (50 μg/ml) was intravenously injected into the mice in each group, respectively. Afterwards, the real-time tomographic images on the spatial level were acquired at 2-11 min, 30 min, 1, 2, 3, 4, 6, 8, 10, 12, and 24 h post-injection, and pharmacokinetic rates were derived for semi-quantitative assessment of the pharmacokinetics of nanoparticles at the tumor site using our proposed pharmacokinetic analysis method. RESULTS: The results obtained from our proposed dynamic DFT experiment demonstrated the distribution of different ICG formulations on the spatial level and enabled the semi-quantitative analysis of the pharmacokinetics of nanoparticles in the tumor tissue. CONCLUSIONS: The obtained pharmacokinetic rates effectively reflected the metabolic processes of nanoparticles in the tumor tissue, which proves to be beneficial for the development of tumor diagnosis and therapy.
PURPOSE: The purpose of this study was to show a systematic strategy for assessing the pharmacokinetics of indocyanine green (ICG)-loaded nanoparticles in the tumor tissue based on a dynamic diffuse fluorescence tomography (DFT) system. PROCEDURES: Twelve-seven-week-old male Balb/c nude mice bearing HepG2/ADR hepatocellular carcinoma were randomly divided into four groups (n = 3 per group). Four hundred microliters of three types of ICG-loaded nanoparticles (content of ICG: 50 μg/ml) and free ICG (50 μg/ml) was intravenously injected into the mice in each group, respectively. Afterwards, the real-time tomographic images on the spatial level were acquired at 2-11 min, 30 min, 1, 2, 3, 4, 6, 8, 10, 12, and 24 h post-injection, and pharmacokinetic rates were derived for semi-quantitative assessment of the pharmacokinetics of nanoparticles at the tumor site using our proposed pharmacokinetic analysis method. RESULTS: The results obtained from our proposed dynamic DFT experiment demonstrated the distribution of different ICG formulations on the spatial level and enabled the semi-quantitative analysis of the pharmacokinetics of nanoparticles in the tumor tissue. CONCLUSIONS: The obtained pharmacokinetic rates effectively reflected the metabolic processes of nanoparticles in the tumor tissue, which proves to be beneficial for the development of tumor diagnosis and therapy.
Authors: Ralf B Schulz; Angelique Ale; Athanasios Sarantopoulos; Marcus Freyer; Eric Soehngen; Marta Zientkowska; Vasilis Ntziachristos Journal: IEEE Trans Med Imaging Date: 2009-11-10 Impact factor: 10.048
Authors: H Shinohara; A Tanaka; T Kitai; N Yanabu; T Inomoto; S Satoh; E Hatano; Y Yamaoka; K Hirao Journal: Hepatology Date: 1996-01 Impact factor: 17.425