| Literature DB >> 30849403 |
Marta Gea1, Sara Bonetta2, Luca Iannarelli3, Andrea Mario Giovannozzi3, Valter Maurino4, Silvia Bonetta1, Vasile-Dan Hodoroaba5, Caterina Armato6, Andrea Mario Rossi3, Tiziana Schilirò1.
Abstract
The aim of this study was to evaluate cytotoxicity (WST-1 assay), LDH release (LDH assay) and genotoxicity (Comet assay) of three engineered TiO2-NPs with different shapes (bipyramids, rods, platelets) in comparison with two commercial TiO2-NPs (P25, food grade). After NPs characterization (SEM/T-SEM and DLS), biological effects of NPs were assessed on BEAS-2B cells in presence/absence of light. The cellular uptake of NPs was analyzed using Raman spectroscopy. The cytotoxic effects were mostly slight. After light exposure, the largest cytotoxicity (WST-1 assay) was observed for rods; P25, bipyramids and platelets showed a similar effect; no effect was induced by food grade. No LDH release was detected, confirming the low effect on plasma membrane. Food grade and platelets induced direct genotoxicity while P25, food grade and platelets caused oxidative DNA damage. No genotoxic or oxidative damage was induced by bipyramids and rods. Biological effects were overall lower in darkness than after light exposure. Considering that only food grade, P25 and platelets (more agglomerated) were internalized by cells, the uptake resulted correlated with genotoxicity. In conclusion, cytotoxicity of NPs was low and affected by shape and light exposure, while genotoxicity was influenced by cellular-uptake and aggregation tendency.Entities:
Keywords: Comet assay; Cytotoxicity; Genotoxic and oxidative damage; Raman spectroscopy; Shape-engineered TiO(2) nanoparticles
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Year: 2019 PMID： 30849403 DOI： 10.1016/j.fct.2019.02.043
Source DB: PubMed Journal: Food Chem Toxicol ISSN： 0278-6915 Impact factor: 6.023