Literature DB >> 3084545

New method of preparing elastase toxoid from Pseudomonas aeruginosa.

K Morihara, J Y Homma.   

Abstract

A new method for the preparation of elastase toxoid of Pseudomonas aeruginosa was developed. A chloroacetyl peptide derivative (CICH2CO-HOLeu-Ala-Gly-NH2; HOLeu, N-hydroxy-L-leucine), an active-site-directed irreversible inhibitor of Pseudomonas aeruginosa elastase, was used to prepare elastase toxoid with or without pretreatment with Formalin and L-lysine. Elastase toxoid thus obtained appeared to be ideal, possessing negligible enzyme activity while retaining full antigenicity and immunogenicity.

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Year:  1986        PMID： 3084545      PMCID： PMC268571          DOI： 10.1128/jcm.23.1.53-55.1986

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.

Authors:  K Weber; J R Pringle; M Osborn
Journal:  Methods Enzymol       Date:  1972       Impact factor: 1.600

3.  Passive hemagglutination reaction test using formalinized sheep erythrocytes treated with tannic acid and coated with proteast or elastase of Pseudomonas aeruginosa.

Authors:  J Y Homma; T Tomiyama; H Sano; Y Hirao; K Saku
Journal:  Jpn J Exp Med       Date:  1975-10

4.  Pseudomonas aeruginosa elastase. Development of a new substrate, inhibitors, and an affinity ligand.

Authors:  N Nishino; J C Powers
Journal:  J Biol Chem       Date:  1980-04-25       Impact factor: 5.157

5.  Effectiveness of immunization with single and multi-component vaccines prepared from a common antigen (OEP), protease and elastase toxoids of Pseudomonas aeruginosa on protection against hemorrhagic pneumonia in mink due to P. aeruginosa.

Authors:  J Y Homma; C Abe; K Tanamoto; Y Hirao; K Morihara; H Tsuzuki; R Yanagawa; E Honda; Y Aoi; Y Fujimoto; M Goryo; N Imazeki; H Noda; A Goda; S Takeuchi; T Ishihara
Journal:  Jpn J Exp Med       Date:  1978-04
  5 in total
  2 in total

1.  Structural gene and complete amino acid sequence of Pseudomonas aeruginosa IFO 3455 elastase.

Authors:  J Fukushima; S Yamamoto; K Morihara; Y Atsumi; H Takeuchi; S Kawamoto; K Okuda
Journal:  J Bacteriol       Date:  1989-03       Impact factor: 3.490

2.  Site-directed mutagenesis of Glu-141 and His-223 in Pseudomonas aeruginosa elastase: catalytic activity, processing, and protective activity of the elastase against Pseudomonas infection.

Authors:  S Kawamoto; Y Shibano; J Fukushima; N Ishii; K Morihara; K Okuda
Journal:  Infect Immun       Date:  1993-04       Impact factor: 3.441
  2 in total

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